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. 2024 Nov 27;11(12):602.
doi: 10.3390/vetsci11120602.

Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa

Martin Ptáček  1 , Filipp Georgijevič Savvulidi  1 , Christopher LeBrun  1 , Martina Janošíková  1 , Temirkhan Kenzhebaev  2 , Kairly Omashev  2 , Beybit Kulataev  2 , Nurlan Malmakov  2
Affiliations

Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa

Martin Ptáček et al. Vet Sci. .

Abstract

Artificial insemination is an advanced reproductive technology used to increase the number of lambs born from elite sires to accelerate genetic gain in a flock [...].

Keywords: CASA; cryopreservation; flow cytometry; insemination doses; ovine; spermatozoa intactness.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Scheme of semen thawing procedure design. T1–5 = thawing temperature (where T1—45 °C; T2—50 °C; T3—55 °C; T4—60 °C; T5—65 °C).
Figure 2
Figure 2
Total (TM) and progressive (PM) motility modified thawing protocols compared to the reference (39 °C/s) one. T0 = results detected immediately after thawing; T2 = results detected after 2 h of incubation in a water bath heated at 39 °C; black points with bounded lines indicate LSM values ± SE for modified thawing protocols; the red line with a grey marked area indicates the reference thawing protocol (LSM ± SE); * = indicates a significant difference in the modified thawing protocol to the reference thawing protocol at p < 0.05 level of significance.
Figure 3
Figure 3
Sperm viability (VIA) of modified thawing protocols compared to the reference (39 °C/s) one. T0 = results detected immediately after thawing; T2 = results detected after 2 h of incubation in a water bath heated at 39 °C; black points with bounded line indicate LSM values ± SE for modified thawing protocols; the red line with a grey marked area indicates the reference thawing protocol (LSM ± SE); * = indicates a significant difference in the modified thawing protocol to reference the thawing protocol at p < 0.05 level of significance.
Figure 4
Figure 4
(A)—Graphical illustration of the thawing curves for different thawing regimes. The temperature change inside the straw during thawing is shown. The red line represents the thawing curve of the control regime (39 degrees for 30 s). (B)—The approximation of thermal energy (calculated in Joules) absorbed by a single straw at different thawing regimes. The red dotted line represents the energy absorbed by a single straw at a reference thawing regime (39 degrees for 30 s).
See this image and copyright information in PMC

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