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. 2024 Dec 20;11(12):670.
doi: 10.3390/vetsci11120670.

Antibacterial Effect of Canine Leucocyte Platelet-Rich Plasma (L-PRP) and Canine Platelet-Poor Plasma (PPP) Against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus pseudintermedius

Roberta Perego  1 , Gabriele Meroni  2 , Piera Anna Martino  2 , Eva Spada  1 , Luciana Baggiani  1 , Daniela Proverbio  1
Affiliations

Antibacterial Effect of Canine Leucocyte Platelet-Rich Plasma (L-PRP) and Canine Platelet-Poor Plasma (PPP) Against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus pseudintermedius

Roberta Perego et al. Vet Sci. .

Abstract

Staphylococcus pseudintermedius (SP) is a commensal and opportunistic pathogen of skin and mucosal surfaces, isolated from healthy dogs and from canine pyoderma cases. It has recently gained attention due to its increasing antibiotic resistance. Platelet-rich plasma (PRP) is a biological product, obtained through a blood centrifugation process, which has antibacterial properties evidenced by in vitro and in vivo studies conducted in both the human and veterinary field. This in vitro study evaluated the antimicrobial effect of canine non-activated and activated leucocyte-rich PRP (L-PRP) and platelet-poor plasma (PPP) against two strains of SP isolated from dogs with pyoderma: one a multidrug-resistant strain (MDR) and one a non-MDR strain. Twenty healthy un-sedated adult blood donor dogs were enrolled for L-PRP and PPP production via a closed semi-automatic system for veterinary use. The evaluation of antimicrobial effect was performed using the micro-inhibition in broth method, exposing SP strains to 10 L-PRP, 10 activated L-PRP and 10 PPP samples, respectively. Bacterial growth was evaluated using CFU count at three timepoints (immediately after incubation T0, after 1 h T1 and after 2 h T2). L-PRP and PPP had a significant antimicrobial effect at all three timepoints which was similar against both non-MDR and MDR SP strains. Activation appeared to reduce the duration of the antimicrobial effect in L-PRP. More studies are necessary to confirm these preliminary results.

Keywords: L-PRP; PPP; S. pseudintermedius; antimicrobial effect; dog.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Antibacterial effect of L-PRP against non-MDR and MDR SP strains. * statistically significant.
Figure 2
Figure 2
Antibacterial effect of PPP against non-MDR and MDR SP strains. * statistically significant.
Figure 3
Figure 3
Effect of animal sex on the antibacterial activity of L-PRP (A,B) and PPP (C,D) against SP strains. * statistically significant.
Figure 4
Figure 4
Activated L- PRP antibacterial action over time for SP40 (A) and SP67 (B) strains.
See this image and copyright information in PMC

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