Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jan;79(1):e70003.
doi: 10.1002/syn.70003.

Synergistic Anxiolytic Effects of Linalool and Sesamol Co-Treatment on Swiss Albino Mice: A Potential GABAergic Intervention

Affiliations

Synergistic Anxiolytic Effects of Linalool and Sesamol Co-Treatment on Swiss Albino Mice: A Potential GABAergic Intervention

Muhammad Torequl Islam et al. Synapse. 2025 Jan.

Abstract

Sesamol (SES) and linalool (LIN) are aromatic compounds that have neuroprotective effects. The main purpose of this study is to evaluate the anxiolytic activity of LIN and SES co-treatment on Swiss albino mice and analyze its possible mechanism through in silico study. In this sense, the mice were given the gamma-aminobutyric acid type A receptors (GABAA) agonist diazepam (DZP; 3 mg/kg, p.o.) as a positive control. A vehicle (10 mL/kg) was served as control. The tested chemicals, single-dose LIN (50 mg/kg) and SES (50 mg/kg), as well as a combination (LIN + SES) and (DZP + LIN + SES), were administered orally in order to conduct several behavioral tests, including open-field, swings box, hole-crossing, and dark-resident time tests. Further, molecular docking studies of LIN, SES, and DZP were carried out through different software. The results showed that LIN and SES individually have significant anxiolytic-like activity in mice. Further, when LIN was combined with SES and with (SES + DZP), it exhibited a relatively lower locomotor activity in mice compared to individual treatment groups, indicating a synergistic action. In addition, the molecular docking analysis revealed that LIN and SES have a moderate binding affinity (-5.0 and -5.1 kcal/mol) toward the GABAA receptor α3 subunit. In conclusion, our findings suggest that LIN and SES exerted synergistic anxiolytic activity on Swiss albino mice, possibly through the GABAergic interaction pathways.

Keywords: GABAA receptor; anxiety; linalool; molecular docking; sesamol.

PubMed Disclaimer

Similar articles

References

    1. Akbor, M. S., M. H. Bappi, A. A. S. Prottay, et al. 2023. “Synergistic Hypnotic Effects of Sesamol and Thymol Possibly Through GABAergic Interaction Pathway: In Vivo and In Silico Studies.” Journal of Biological Regulators and Homeostatic Agents 37, no. 11: 6419–6435.
    1. Aman, U., F. Subhan, M. Shahid, et al. 2016. “Passiflora Incarnata Attenuation of Neuropathic Allodynia and Vulvodynia Apropos GABA‐Ergic and Opioidergic Antinociceptive and Behavioural Mechanisms.” BMC Complementary and Alternative Medicine 16: 1–17.
    1. de Moura do Amaral, M. P., M. P. da Silva Junior, F. das Chagas Alves Lima, S. J. C. Gutierrez, D. D. R. Arcanjo, and R. D. C. M. Oliveira. 2023. “Anxiolytic/Sedative Effect of Monoterpene (–)‐Borneol in Mice and In Silico Molecular Interaction With GABAA Receptor.” Future Pharmacology 3, no. 1: 132–141.
    1. Aprotosoaie, A. C., M. Hăncianu, I. I. Costache, and A. Miron. 2014. “Linalool: A Review on a Key Odorant Molecule With Valuable Biological Properties.” Flavour and Fragrance Journal 29, no. 4: 193–219.
    1. Atack, J. R. 2005. “The Benzodiazepine Binding Site of GABAA Receptors as a Target for the Development of Novel Anxiolytics.” Expert Opinion on Investigational Drugs 14, no. 5: 601–618.

Grants and funding

LinkOut - more resources