Standard: Human gastric cancer organoids

Ronghui Tan^#¹, Fan Hong^#¹, Ting Wang^#^{2

3}, Nanshan Zhong^#⁴, Hongling Zhao³, Rui-Hua Xu^{5

6}, Lin Shen⁷, Yingbin Liu^{8

9}, Xuebiao Yao¹⁰, Dongxi Xiang^{8

9}, Dong Gao^{11

12

13}, Jianping Xiong^{14

15}, Lijian Hui^{12

16

17}, Bing Zhao⁴, Zhifeng Miao^{18

19

20}, Jie Hao^{21

22}, Yong Li²³, Shijun Hu²⁴, Boqiang Fu²⁵, Guoqiang Hua^{26

27}, Lei Wang^{3

21}, Zhao-Lei Zeng⁵, Chong Chen²⁸, Jianmin Wu^{29

30}, Changlin Wang^{31

32}, Chunnian Wang³³, Xianbao Zhan³⁴, Chen Song³⁵, Zhijian Sun³⁶, Chunping Yu³⁷, Yingying Yang³⁸, Gengming Niu³⁹, Yalong Wang⁴⁰, Tongbiao Zhao^{41

42

43}, Ye-Guang Chen^{44

45

46}

Affiliations

¹ Guangzhou National Laboratory, Guangzhou, 510005, China.
² The State Key Laboratory of Membrane Biology, Tsinghua‑Peking Center for Life Sciences,, School of Life Sciences , Tsinghua University, Beijing, 100084, China.
³ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China.
⁴ The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China.
⁵ State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, China.
⁶ Research Unit of Precision Diagnosis and Treatment for Gastrointestinal Cancer, Chinese Academy of Medical Sciences, Guangzhou, 510060, China.
⁷ State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers, Department of Gastrointestinal Oncology, Beijing Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing, 100142, China.
⁸ State Key Laboratory of Systems Medicine for Cancer, Shanghai Cancer Institute, Shanghai Jiaotong University School of Medicine, Shanghai, 200232, China.
⁹ Department of Biliary-Pancreatic Surgery, Renji HospitalAffiliated to, Shanghai Jiaotong University School of Medicine , Shanghai, 200127, China.
¹⁰ MOE Key Laboratory of Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, University of Science and Technology of China, Hefei, 230027, China.
¹¹ Key Laboratory of Multi-Cell Systems, Shanghai Key Laboratory of Molecular Andrology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China.
¹² University of Chinese Academy of Sciences, Beijing, 100049, China.
¹³ Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen, 518132, China.
¹⁴ Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330031, China.
¹⁵ Jiangxi Key Laboratory for Individual Cancer Therapy, Nanchang, Jiangxi, 330031, China.
¹⁶ School of Life Science and Technology, Shanghai Tech University, Shanghai, 201210, China.
¹⁷ State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Science, Shanghai, 200031, China.
¹⁸ Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, 155N Nanjing Street, Shenyang, Liaoning, 110001, China.
¹⁹ Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, China Medical University, Shenyang, Liaoning, 110001, China.
²⁰ Institute of Health Sciences, China Medical University, Shenyang, Liaoning, 110001, China.
²¹ National Stem Cell Resource Center, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
²² State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
²³ Department of Gastrointestinal Surgery, Department of General Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510080, China.
²⁴ Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Suzhou, 510080, China.
²⁵ National Institute of Metrology, Beijing, 100029, China.
²⁶ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200433, China.
²⁷ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China.
²⁸ Gastric Cancer Centerand, Laboratory of Gastric Cancer, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
²⁹ Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Center for Cancer Bioinformatics, Peking University Cancer Hospital & Institute, Beijing, 100142, China.
³⁰ Peking University International Cancer Institute, Peking University, Beijing, 100191, China.
³¹ China National Institute of Standardization, Beijing, 100191, China.
³² Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China.
³³ Department of Gastrointestinal Pathology, Ningbo Diagnostic Pathology Center, Ningbo, 315021, China.
³⁴ Department of Oncology, Changhai Hospital, Second Military Medical University, No. 168 Changhai Road, Shanghai, 200433, China.
³⁵ Huayi Regeneration Technology Co., Ltd, Chengdu, 611135, China.
³⁶ K2 Oncology Co., Ltd, KeChuang Street, Beijing, 100176, China.
³⁷ Lilly (China) Research and Development Center, Shanghai, 201203, China.
³⁸ Qilu Pharmaceutical Co., Ltd, Jinan, 250104, China.
³⁹ Shanghai OneTar Biomedicine Co., Ltd, Shanghai, 201203, China.
⁴⁰ Guangzhou National Laboratory, Guangzhou, 510005, China. wangyl16@tsinghua.org.cn.
⁴¹ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴² National Stem Cell Resource Center, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴³ State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴⁴ Guangzhou National Laboratory, Guangzhou, 510005, China. ygchen@tsinghua.edu.cn.
⁴⁵ The State Key Laboratory of Membrane Biology, Tsinghua‑Peking Center for Life Sciences,, School of Life Sciences , Tsinghua University, Beijing, 100084, China. ygchen@tsinghua.edu.cn.
⁴⁶ The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China. ygchen@tsinghua.edu.cn.

^# Contributed equally.

PMID: 39729207
PMCID: PMC11680512
DOI: 10.1186/s13619-024-00217-7

Editorial

Standard: Human gastric cancer organoids

Ronghui Tan et al. Cell Regen. 2024.

. 2024 Dec 27;13(1):33.

doi: 10.1186/s13619-024-00217-7.

Authors

Affiliations

¹ Guangzhou National Laboratory, Guangzhou, 510005, China.
² The State Key Laboratory of Membrane Biology, Tsinghua‑Peking Center for Life Sciences,, School of Life Sciences , Tsinghua University, Beijing, 100084, China.
³ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China.
⁴ The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China.
⁵ State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, China.
⁶ Research Unit of Precision Diagnosis and Treatment for Gastrointestinal Cancer, Chinese Academy of Medical Sciences, Guangzhou, 510060, China.
⁷ State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers, Department of Gastrointestinal Oncology, Beijing Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing, 100142, China.
⁸ State Key Laboratory of Systems Medicine for Cancer, Shanghai Cancer Institute, Shanghai Jiaotong University School of Medicine, Shanghai, 200232, China.
⁹ Department of Biliary-Pancreatic Surgery, Renji HospitalAffiliated to, Shanghai Jiaotong University School of Medicine , Shanghai, 200127, China.
¹⁰ MOE Key Laboratory of Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, University of Science and Technology of China, Hefei, 230027, China.
¹¹ Key Laboratory of Multi-Cell Systems, Shanghai Key Laboratory of Molecular Andrology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China.
¹² University of Chinese Academy of Sciences, Beijing, 100049, China.
¹³ Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen, 518132, China.
¹⁴ Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330031, China.
¹⁵ Jiangxi Key Laboratory for Individual Cancer Therapy, Nanchang, Jiangxi, 330031, China.
¹⁶ School of Life Science and Technology, Shanghai Tech University, Shanghai, 201210, China.
¹⁷ State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Science, Shanghai, 200031, China.
¹⁸ Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, 155N Nanjing Street, Shenyang, Liaoning, 110001, China.
¹⁹ Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, China Medical University, Shenyang, Liaoning, 110001, China.
²⁰ Institute of Health Sciences, China Medical University, Shenyang, Liaoning, 110001, China.
²¹ National Stem Cell Resource Center, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
²² State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
²³ Department of Gastrointestinal Surgery, Department of General Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510080, China.
²⁴ Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Suzhou, 510080, China.
²⁵ National Institute of Metrology, Beijing, 100029, China.
²⁶ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200433, China.
²⁷ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China.
²⁸ Gastric Cancer Centerand, Laboratory of Gastric Cancer, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
²⁹ Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Center for Cancer Bioinformatics, Peking University Cancer Hospital & Institute, Beijing, 100142, China.
³⁰ Peking University International Cancer Institute, Peking University, Beijing, 100191, China.
³¹ China National Institute of Standardization, Beijing, 100191, China.
³² Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China.
³³ Department of Gastrointestinal Pathology, Ningbo Diagnostic Pathology Center, Ningbo, 315021, China.
³⁴ Department of Oncology, Changhai Hospital, Second Military Medical University, No. 168 Changhai Road, Shanghai, 200433, China.
³⁵ Huayi Regeneration Technology Co., Ltd, Chengdu, 611135, China.
³⁶ K2 Oncology Co., Ltd, KeChuang Street, Beijing, 100176, China.
³⁷ Lilly (China) Research and Development Center, Shanghai, 201203, China.
³⁸ Qilu Pharmaceutical Co., Ltd, Jinan, 250104, China.
³⁹ Shanghai OneTar Biomedicine Co., Ltd, Shanghai, 201203, China.
⁴⁰ Guangzhou National Laboratory, Guangzhou, 510005, China. wangyl16@tsinghua.org.cn.
⁴¹ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴² National Stem Cell Resource Center, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴³ State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴⁴ Guangzhou National Laboratory, Guangzhou, 510005, China. ygchen@tsinghua.edu.cn.
⁴⁵ The State Key Laboratory of Membrane Biology, Tsinghua‑Peking Center for Life Sciences,, School of Life Sciences , Tsinghua University, Beijing, 100084, China. ygchen@tsinghua.edu.cn.
⁴⁶ The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China. ygchen@tsinghua.edu.cn.

^# Contributed equally.

PMID: 39729207
PMCID: PMC11680512
DOI: 10.1186/s13619-024-00217-7

Abstract

Gastric cancer is one of the most common malignancies with poor prognosis. The use of organoids to simulate gastric cancer has rapidly developed over the past several years. Patient-derived gastric cancer organoids serve as in vitro models that closely mimics donor characteristics, offering new opportunities for both basic and applied research. The "Human Gastric Cancer Organoid" is part of a series of guidelines for human gastric cancer organoids in China, jointly drafted by experts from the Chinese Society for Cell Biology and its branches, and initially released on October 29, 2024. This standard outlines terminology, technical requirements, assessment protocols, and applies to production, evaluation procedures, and quality control for human gastric cancer organoids. The publication of this guideline aims to assist institutions in endorsing, establishing, and applying best practices, advancing the international standardization of human gastric cancer organoids for clinical development and therapeutic application.

Keywords: Definitions; Gastric cancer organoids; Human stomach; Labeling; Standard; Storage; Technical requirements; Terms; Transportation.

PubMed Disclaimer

Conflict of interest statement

Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: Y.-G.C. is the Editor‑in‑Chief of Cell Regeneration. He was not involved in the review or decision related to this manuscript. This work was not sponsored by any commercial organizations, and all the other authors declare that they have no competing interests.

References

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Standard: Human gastric cancer organoids

Affiliations

Standard: Human gastric cancer organoids

Authors

Affiliations

Abstract

Conflict of interest statement

References

Publication types

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