Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025:2892:211-231.
doi: 10.1007/978-1-0716-4330-3_15.

Application of Proteomic Methods in Oomycete Biology

Christina E Andronis  1   2 , Scott Bringans  1 , Kar-Chun Tan  3
Affiliations

Application of Proteomic Methods in Oomycete Biology

Christina E Andronis et al. Methods Mol Biol. 2025.

Abstract

The biochemical makeup of any organism provides insight into key factors regarding its biological functions. These factors can be explored using proteomics, which allows us to obtain a snapshot of the protein content and abundance in an organism, cell type or sub-cellular compartment. Here, we describe proteomic methodologies that can be used to dissect the biochemical mechanism of phytopathogenicity in oomycetes. These methodologies include protein extraction, purification, subsequent processing, mass spectrometry analysis, and qualitative and quantitative data processing of oomycete proteomes for comparative studies. Additionally, the use of mass spectra to assist in gene validation and modelling in unfinished oomycete genomes is also described.

Keywords: Mass spectrometry; Oomycete; Proteogenomics; Proteome; Proteomics.

PubMed Disclaimer

References

    1. Aslam B, Basit M, Nisar MA et al (2017) Proteomics: technologies and their applications. J Chromatogr Sci 55:182–196. https://doi.org/10.1093/chromsci/bmw167 - DOI - PubMed
    1. Shepherd SJ, van West P, Gow NA (2003) Proteomic analysis of asexual development of Phytophthora palmivora. Mycol Res 107:395–400. https://doi.org/10.1017/S0953756203007561 - DOI - PubMed
    1. Pang Z, Srivastava V, Liu X, Bulone V (2016) Quantitative proteomics links metabolic pathways to specific developmental stages of the plant pathogenic oomycete Phytophthora capsici. Mol Plant Pathol. https://doi.org/10.1111/mpp.12406
    1. Resjö S, Ali A, Meijer HJG et al (2014) Quantitative label-free phosphoproteomics of six different life stages of the late blight pathogen Phytophthora infestans reveals abundant phosphorylation of members of the CRN effector family. J Proteome Res 13:1848–1859. https://doi.org/10.1021/pr4009095 - DOI - PubMed
    1. Hosseini S, Resjö S, Liu Y et al (2015) Comparative proteomic analysis of hyphae and germinating cysts of Phytophthora pisi and Phytophthora sojae. J Proteome 117:24–40. https://doi.org/10.1016/j.jprot.2015.01.006 - DOI

LinkOut - more resources