Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Feb;21(2):249-50.
doi: 10.1128/jcm.21.2.249-250.1985.

Radioenzymatic assay for trimethoprim in very small serum samples

R Yogev, C Melick, L Tan-Pong

Radioenzymatic assay for trimethoprim in very small serum samples

R Yogev et al. J Clin Microbiol. 1985 Feb.

Abstract

A modification of the methotrexate radioassay kit (supplied by New England Enzyme Center) enabled determination of trimethoprim levels in 5-microliter serum samples. An excellent correlation between this assay and high-pressure liquid chromatography assay was found. These preliminary results suggest that with this method rapid determination of trimethoprim levels in very small samples (5 to 10 microliters) can be achieved.

PubMed Disclaimer

References

    1. N Engl J Med. 1977 Jan 6;296(1):47-8 - PubMed
    1. J Chromatogr. 1983 Dec 9;278(2):337-45 - PubMed
    1. Antimicrob Agents Chemother. 1979 Nov;16(5):579-83 - PubMed