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. 1985 Feb 11;76(2):389-93.
doi: 10.1016/0022-1759(85)90316-3.

Enzyme amplification--a general method applied to provide an immunoassisted assay for placental alkaline phosphatase

C H Self

Enzyme amplification--a general method applied to provide an immunoassisted assay for placental alkaline phosphatase

C H Self. J Immunol Methods. .

Abstract

A method is described in which enzyme amplification is used for the quantitative determination of placental alkaline phosphatase. In this particular application, placental alkaline phosphatase is captured on a solid surface by means of a monoclonal antibody. The enzyme is then determined by dephosphorylation of NADP to NAD which activates a strictly NAD-dependent redox cycle leading to the formation of a coloured product.

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