doi: 10.1016/0022-1759(85)90316-3.
Enzyme amplification--a general method applied to provide an immunoassisted assay for placental alkaline phosphatase
- PMID: 3973395
- DOI: 10.1016/0022-1759(85)90316-3
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Enzyme amplification--a general method applied to provide an immunoassisted assay for placental alkaline phosphatase
J Immunol Methods.
.
Abstract
A method is described in which enzyme amplification is used for the quantitative determination of placental alkaline phosphatase. In this particular application, placental alkaline phosphatase is captured on a solid surface by means of a monoclonal antibody. The enzyme is then determined by dephosphorylation of NADP to NAD which activates a strictly NAD-dependent redox cycle leading to the formation of a coloured product.
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