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Comparative Study
. 2024 Dec 30;14(1):31731.
doi: 10.1038/s41598-024-82773-1.

Comparative analysis of TNF-α (-308) gene polymorphism in Turkish patients with type 2 diabetes developing nephropathy and/or neuropathy

Venhar Gurbuz Can  1 , Ozlem Cesur Gunay  2 , Sinem Cesur  3
Affiliations
Comparative Study

Comparative analysis of TNF-α (-308) gene polymorphism in Turkish patients with type 2 diabetes developing nephropathy and/or neuropathy

Venhar Gurbuz Can et al. Sci Rep. .

Abstract

Type 2 diabetes (T2DM) is a group of chronic and systemic metabolic diseases. Nephropathy (NP) and neuropathy (NR) are two common complications that severely affect the quality of life of patients with T2DM. In this study, our aim was to investigate the association between TNF-alpha (-308) gene polymorphism and the risk of developing NP and NR in patients with T2DM We also aimed to determine the association between TNF-alpha (-308) gene polymorphism and several demographic characteristics and biochemical parameters of patients with T2DM developed NP and/or NR. First, blood samples of patients were collected, and DNA isolation was performed. Then, real-time PCR analysis was used for genotyping the TNF-α (-308) gene utilising fluorescently labelled probes. Patients' demographical data and biochemical parameters were also obtained. Our study did not detect any significant difference in the frequency of possessing the gene polymorphism (A allele) between the groups. Additionally, no significant difference was detected when the data on various biochemical parameters were evaluated in GG, GA, and AA genotypes.

Keywords: DNA polymorphism; Nephropathy; Neuropathy; TNF-α; Type 2 diabetes.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
The images of the RT-PCR analysis.
See this image and copyright information in PMC

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References

    1. AssociationA.D. Diagnosis and classification of diabetes Mellitus. Diabetes Care. 33 (Supplement_1), pS62–S69. 10.2337/dc10-S062 (2010).
    1. Defronzo, R. A. Banting lecture. From the triumvirate to the ominous octet: a new paradigm for the treatment of type 2 diabetes mellitus. Diabetes58 (4), 773–795. 10.2337/db09-9028 (2009). - PMC - PubMed
    1. Tartaglia, L. A. et al. The two different receptors for tumor necrosis factor mediate distinct cellular responses. Proceedings of the National Academy of Sciences, 88(20): pp. 9292–9296. DOI: doi: (1991). 10.1073/pnas.88.20.9292 - PMC - PubMed
    1. Peng, Y. & Li, L. J. TNF-α-308G/A polymorphism associated with TNF-α protein expression in patients with diabetic nephropathy. Int. J. Clin. Exp. Pathol.8 (3), 3127–3131 (2015). - PMC - PubMed
    1. Rizvi, S., Raza, S. T. & Mahdi, F. Association of genetic variants with diabetic nephropathy. World J. Diabetes. 5 (6), 809–816. 10.4239/wjd.v5.i6.809 (2014). - PMC - PubMed

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