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. 2024 Dec 30;41(1):22.
doi: 10.1007/s11274-024-04236-z.

Multilocus sequencing analysis of the rhizobial symbionts isolated from Acacia salicina (Lindl.) grown in different regions in Tunisia reveals putative novel Bradyrhizobium species

Mokhtar Rejili  1   2 , Besma Bouznif  3 , Mohamed Ali Benabderrahim  4   5 , Mohamed Mars  3
Affiliations

Multilocus sequencing analysis of the rhizobial symbionts isolated from Acacia salicina (Lindl.) grown in different regions in Tunisia reveals putative novel Bradyrhizobium species

Mokhtar Rejili et al. World J Microbiol Biotechnol. .

Abstract

In this study, we investigated various chromosomal and symbiotic markers in 40 bacterial strains that nodulate an invasive alien plant, Acacia salicina Lindl. in Tunisia. Our findings showed that the native rhizobia associated to A. salicina are grouped into eight distinct RAPD electrophoretic types (RETs) (genotypes). Sequence analyses of rrs gene and three housekeeping genes (recA, rpoB and glnII) assigned sixteen isolates to three putative new lineages within the genus Bradyrhizobium. Seven strains clustered with B. rifense CTAW71T with a 91% bootstrap support, five strains grouped with B. niftali CNPSo3448T with a very low bootstrap support (60%), and four strains formed a group phylogenetically related with B. shewense ERR11T and B. centrosematis A9T. Based on nodC phylogeny and cross inoculation tests, the 16 strains are clustered within symbiovar retamae (six strains) and cyanophyllae (ten strains). Moreover, we showed by the first time in this work that the type strains B. diversitatis CNPSo4019T and B. xenonodulans 14ABT, which nodulated soybean and A. dealbata respectively, belong to the symbiovar cyanophyllae according to the results of the nodC gene analysis.

Keywords: Acacia salicina; Bradyrhizobium; Chromosomal ligneages; Tunisia.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare no competing interests. Ethical approval: This article did not contain any studies with animals performed by any of the authors.

References

    1. Andrews M, De Meyer S, James EK, Stępkowski T, Hodge S, Simon MF, Young JPW (2018) Horizontal transfer of symbiosis genes within and between rhizobial genera: occurrence and importance. Genes 9:321. https://doi.org/10.3390/genes9070321 - DOI - PubMed - PMC
    1. Ahnia H, Bourebaba Y, Durán D, Boulila F et al (2018) Bradyrhizobium algeriense sp. nov., a novel species isolated from effective nodules of Retama sphaerocarpa from Northeastern Algeria. Syst Appl Microbiol 41:333–339. https://doi.org/10.1016/j.syapm.2018.03.004 - DOI - PubMed
    1. Aserse AA, Woyke T, Kyrpides NC, Whitman WB, Lindström K (2017) Draft genome sequence of type strain HBR26T and description of Rhizobium aethiopicum sp. nov. Stand Genomic Sci 12:1–16. https://doi.org/10.1186/s40793-017-0220-z - DOI
    1. Ba S, Willems A, de Lajudie P, Roche P et al (2002) Symbiotic and taxonomic diversity of rhizobia isolated from Acacia tortilis subsp. raddiana in Africa. Syst Appl Microbiol 25:130–145. https://doi.org/10.1078/0723-2020-00091 - DOI - PubMed
    1. Baele M, Baele P, Vaneechoutte M, Storms V et al (2000) Application of tRNA inter-genic spacer PCR for identification of Enterococcus species. J Clin Microbiol 38:4201–4207. https://doi.org/10.1128/JCM.38.11.4201-4207.2000 - DOI - PubMed - PMC

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