Plant extracellular vesicles contribute to the amplification of immune signals during systemic acquired resistance

Abstract

Plant extracellular vesicles play a role in systemic acquired resistance by facilitating the transmission of immune signals between plant cells. Extracellular vesicles (EVs) play a critical role in facilitating the transfer of nucleic acids and proteins between plants and pathogens. However, the involvement of plant EVs in intercellular communication and their contribution to the regulation of physiological and pathological conditions in plants remains unclear. In this study, we isolated EVs from the apoplast of Arabidopsis plants induced by systemic acquired resistance (SAR) and conducted proteomic and physiological analyses to investigate the role of EVs in SAR. The results demonstrated that plant cells are capable of internalizing EVs, and EV secretion was enhanced in SAR-induced plants. EVs isolated from SAR-induced plants effectively inhibited the spore production of Botrytis cinerea, activated the transcription of several SAR marker genes, and improved plant resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). Several proteins associated with defense responses were enriched in EVs upon SAR induction. Among these, the receptor-like kinase H₂O₂-Induced Ca²⁺ Increase 1 (HPCA1) was identified as a crucial component in SAR. In addition, plant EVs contained numerous proteins involved in the transmission of signals related to pathogen-associated molecular patterns-triggered immunity (PTI) and effector-triggered immunity (ETI). Our findings suggest that plant EVs are functionally involved in the propagation of SAR signals and may play diverse roles in plant immune responses.

Keywords: Arabidopsis thaliana; Defense response; Extracellular vesicles; Plant immunity; Proteomics; Systemic acquired resistance.