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. 2024 Dec 30.
doi: 10.1007/s12602-024-10440-y. Online ahead of print.

Bacillus licheniformis B410 Alleviates Inflammation Induced by Lipopolysaccharide and Salmonella by Inhibiting NF-κB and IRF Signaling Pathways in Macrophages

Dan Xiong  1   2   3 , Tao Sun  1   3 , Mengru Liu  1   3 , Bo Wang  1   3 , Tianzhu Guan  1   3 , Li Song  2   4 , Xinan Jiao  2 , Zhenquan Yang  5   6   7
Affiliations

Bacillus licheniformis B410 Alleviates Inflammation Induced by Lipopolysaccharide and Salmonella by Inhibiting NF-κB and IRF Signaling Pathways in Macrophages

Dan Xiong et al. Probiotics Antimicrob Proteins. .

Abstract

Foodborne bacterial enteritis is a common clinical disease, and its incidence has risen globally. To screen for functional Bacillus strains with anti-inflammatory properties, tolerance to acid and bile salts, and antagonism against Salmonella, 22 strains of Bacillus were employed as candidate strains in this study. An inflammatory cell model was established using J774-Dual NF-κB/IRF reporter macrophages to identify anti-inflammatory Bacillus. The candidate Bacillus strains were assessed through tolerance to acid and bile salts and antibacterial assays, and their inhibitory effects on the inflammatory responses triggered by S. Enteritidis infection were investigated. The findings demonstrated that B. licheniformis B410 was successfully screened, possessing a significant anti-inflammatory effect by suppressing the NF-κB and IRF signaling pathways. B410 exhibited excellent tolerance to acid and bile salts and displayed a favorable antibacterial effect against Salmonella. Co-incubation of B410 with RAW264.7 macrophages did not influence the cell viability. B. licheniformis B410 could significantly inhibit the expression of pro-inflammatory cytokines IL-1β and TNF-α induced by LPS and promote the expression of the anti-inflammatory cytokine IL-10. Additionally, B410 could markedly inhibit the activation of NF-κB and the production of inflammatory cytokines caused by S. Enteritidis infection in macrophages. This study successfully screened a new strain of B. licheniformis B410 that simultaneously had the capabilities of anti-inflammation, acid and bile salt tolerance, and antagonism against Salmonella, providing a new approach for the screening of functional anti-inflammatory probiotics and the development of anti-inflammatory probiotic therapeutic preparations.

Keywords: Bacillus; Salmonella; Acid and bile salt tolerance; Anti-inflammation; IRF signaling pathway; NF-κB signaling pathway.

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Conflict of interest statement

Declarations. Competing Interests: The authors declare no competing interests.

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