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. 2025 Jan 1;16(2):498-505.
doi: 10.7150/jca.103945. eCollection 2025.

The association between the polymorphism of RAR related orphan receptor beta and the clinical manifestations of oral squamous cell carcinoma

Wei-En Yang  1   2 , Chiao-Wen Lin  3   4 , Yi-Tzu Chen  3   4 , Chun-Wen Su  1   2 , Chia-Ming Yeh  1   2 , Chia-Yi Lee  1   5 , Shun-Fa Yang  1   2 , Mu-Kuan Chen  6   7
Affiliations

The association between the polymorphism of RAR related orphan receptor beta and the clinical manifestations of oral squamous cell carcinoma

Wei-En Yang et al. J Cancer. .

Abstract

Oral squamous cell carcinoma (OSCC) affects a substantial proportion of the Asian population and is influenced by various genetic risk factors. The RAR-related orphan receptor beta (RORB), a regulator of the circadian rhythm, has been implicated in certain neoplasms. Accordingly, this study investigated the association between RORB single-nucleotide polymorphisms and clinical manifestations of OSCC. A total of 1174 male patients without OSCC and 1254 male patients with OSCC were included in the study. Three RORB single-nucleotide polymorphism loci-rs3750420 (T/C), rs10781247 (A/G), and rs17611535 (C/T)-were genotyped using TaqMan allelic discrimination assays. RORB single-nucleotide polymorphism rs10781247 variants were significantly associated with moderate to poor cellular differentiation in patients with OSCC (p = 0.042). Additionally, among betel quid chewers with OSCC, rs10781247 variants were significantly associated with moderate to poor cell differentiation (p = 0.036). The rs3750420 variants were significantly associated with larger tumor size in individuals with buccal mucosa cancer (p = 0.036). An analysis of Cancer Genome Atlas data revealed that RORB mRNA levels were significantly higher in patients with head and neck squamous cell carcinoma compared with controls (p = 0.0002). Moreover, RORB mRNA levels were significantly higher in stage IV tumors than in stage III tumors (p = 0.0252). In conclusion, RORB single-nucleotide polymorphisms rs3750420 and rs10781247 are associated with adverse clinical characteristics in OSCC.

Keywords: RAR related orphan receptor beta; betel quid chewing; buccal mucosa cancer; oral cancer; oral squamous cell carcinoma; single nucleotide polymorphism.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
The Analysis of RORB mRNA by The Cancer Genome Atlas database. (A) The RORB mRNA level in head and neck squamous cell carcinoma patients and noncancerous tissues. (B) The RORB mRNA level in head and neck squamous cell carcinoma patients with different tumor stage.
See this image and copyright information in PMC

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