Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025:2889:107-119.
doi: 10.1007/978-1-0716-4322-8_8.

Studying the Role of HOX Genes in Thrombocyte Development

Jabila Mary  1 , Pudur Jagadeeswaran  2
Affiliations

Studying the Role of HOX Genes in Thrombocyte Development

Jabila Mary et al. Methods Mol Biol. 2025.

Abstract

In our laboratory, we study thrombopoiesis and hemostasis using zebrafish as a model organism to unravel the mechanisms of differentiation and development of thrombocytes. We have shown in our earlier work that thrombocytes are functional equivalents of platelets and have transcriptional machinery similar to megakaryocytes. We recently found evidence that hox genes play a role in their development. We used piggyback gene knockdown and thrombocyte quantification assays to understand the influence of these ancient developmental regulators on thrombopoiesis. In this chapter, we describe methods used to discover these hox genes.

Keywords: DiI labeling; Flow cytometry; Piggyback knockdown; RT-PCR; Thrombocytes; qRT-PCR.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Lappin TR, Grier DG, Thompson A, Halliday HL (2006) HOX genes: seductive science, mysterious mechanisms. Ulster Med J 75(1):23–31 - PubMed - PMC
    1. Lewis EB (1978) A gene complex controlling segmentation in Drosophila. Nature 276(5688):565–570. https://doi.org/10.1038/276565a0 - DOI - PubMed
    1. Amores A et al (1998) Zebrafish hox clusters and vertebrate genome evolution. Science 282(5394):1711–1714. https://doi.org/10.1126/science.282.5394.1711 - DOI - PubMed
    1. Zhong Y, Sullenbarger B, Lasky LC (2010) Effect of increased HoxB4 on human megakaryocytic development. Biochem Biophys Res Commun 398(3):377–382. https://doi.org/10.1016/j.bbrc.2010.06.075 - DOI - PubMed - PMC
    1. Kaushansky K (2005) Thrombopoietin and the hematopoietic stem cell. Ann N Y Acad Sci 1044:139–141. https://doi.org/10.1196/annals.1349.018 - DOI - PubMed

MeSH terms

LinkOut - more resources