Protein coding assignment of the S genes of the avian reovirus S1133

Abstract

The protein coding assignments for each of the S genes of the avian reovirus S1133 have been determined. In vitro translation of RNA derived from individual S dsRNA genome segments demonstrated that the largest S gene, S1, codes for the smallest protein, sigma C; the S2 for sigma A; the S3 gene for sigma C; and the S4 gene for sigma NS. No other gene products could be identified. By examination of appropriate reassortant viruses, these assignments were confirmed.