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. 2024 Dec 21;15(12):1640.
doi: 10.3390/genes15121640.

Impact of Long-Term Cannabidiol (CBD) Treatment on Mouse Kidney Transcriptome

Mikołaj Rokicki  1 , Jakub Żurowski  1 , Sebastian Sawicki  2 , Ewa Ocłoń  3 , Tomasz Szmatoła  1 , Igor Jasielczuk  1 , Karolina Mizera-Szpilka  4 , Ewelina Semik-Gurgul  5 , Artur Gurgul  1
Affiliations

Impact of Long-Term Cannabidiol (CBD) Treatment on Mouse Kidney Transcriptome

Mikołaj Rokicki et al. Genes (Basel). .

Abstract

Background: Cannabidiol, which is one of the main cannabinoids present in Cannabis sativa plants, has been shown to have therapeutic properties, including anti-inflammatory and antioxidant effects that may be useful for treatment of various kidney conditions.

Objectives: This article investigates the effect of long-term cannabidiol (CBD) treatment on changes in the renal transcriptome in a mouse model. The main hypothesis was that systematic CBD treatment would affect gene expression associated with those processes in the kidney.

Methods: The study was conducted on male C57BL/6J mice. Mice in the experimental groups received daily intraperitoneal injections of CBD at doses of 10 mg/kg or 20 mg/kg body weight (b.w.) for 28 days. After the experiment, kidney tissues were collected, RNA was isolated, and RNA-Seq sequencing was performed.

Results: The results show CBD's effects on changes in gene expression, including the regulation of genes related to circadian rhythm (e.g., Ciart, Nr1d1, Nr1d2, Per2, and Per3), glucocorticoid receptor function (e.g., Cyp1b1, Ddit4, Foxo3, Gjb2, and Pck1), lipid metabolism (e.g., Cyp2d22, Cyp2d9, Decr2 Hacl1, and Sphk1), and inflammatory response (e.g., Cxcr4 and Ccl28).

Conclusions: The obtained results suggest that CBD may be beneficial for therapeutic purposes in treating kidney disease, and its effects should be further analyzed in clinical trials.

Keywords: CBD; RNA-seq; cannabidiol; kidney; mouse model.

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Conflict of interest statement

All authors declare that have no competing interests.

Figures

Figure 1
Figure 1
Results of RNA-Seq data analysis. (A) Principal components analysis for all samples; (B) number of genes altered with both CBD treatments; (C) MA plots; (D) heatmaps for genes significantly altered using CBD treatments in kidneys.
See this image and copyright information in PMC

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