Correction: Hazzan et al. Thymic Stromal Lymphopoietin Interferes with the Apoptosis of Human Skin Mast Cells by a Dual Strategy Involving STAT5/Mcl-1 and JNK/Bcl-xL. Cells 2019, 8, 829

Abstract

Error in Figure 3 [...].

Figure 3

MC maintenance by TSLP critically depends on JNK and STAT5 activation. Impact of (a,c) STAT5 and (b,d) JNK perturbation on TSLP-promoted MC recovery (at 7.5 ng/mL) after 8 h, evaluated by the ratio of YoPro^TM-1 positivity (corresponding to the percentage of early and late apoptotic/necrotic cells combined) in TSLP-treated versus untreated MCs (described in methods). (a,b) Interference by Accell^®-mediated RNAi (48 h prior to TSLP treatment); (c,d) interference by specific inhibitors (STAT5 inhibitor: pimozide, JNK inhibitor: SP600125). Top: the results represent the mean ± SEM of six independent experiments. Bottom: representative flow cytometry dot plots (specified in red is the percentage of early and late apoptotic/necrotic cells combined); w/o—without. The data were analyzed by paired t-test, ** p < 0.01, *** p < 0.001.

Figure 4

TSLP up-regulates Mcl-1 and Bcl-x_L. TSLP-induced expression (at 7.5 ng/mL) was studied by (a,b) reverse transcription - quantitative polymerase chain reaction (RT-qPCR) analysis of (a) Mcl-1 and (b) Bcl-x_L; normalized to the housekeeping gene Cyclophilin B. The results represent the mean ± SEM of nine independent experiments. The data were analyzed by the one-way Anova test with Tukey’s post-test for multiple comparisons, comparing each treatment (40′ or 90′) with the respective control group; * p < 0.05, ** p < 0.01; and (c,d) Western blot analysis using the indicated antibodies (shown are representative Western blots out of three independent experiments); the anti-β-Actin antibody served as loading control. Densitometry arbitrary units were normalized to the housekeeping protein.

Figure 5

Survival prolongation by TSLP depends on Mcl-1 and Bcl-x_L. Impact of Mcl-1 and Bcl-x_L knockdown on TSLP-promoted MC recovery (at 7.5 ng/mL), as evaluated by apoptosis reduction in TSLP-treated versus untreated MCs after 8 h. (a) Reduction of YoPro^TM-1-positivity (corresponding to the percentage of early and late apoptotic/necrotic cells combined) as mean ± SEM of nine independent experiments (left) and representative flow cytometry dot plots (right) (specified in red is the percentage of early and late apoptotic/necrotic cells combined); w/o—without; (b) reduction of caspase-3 activity as mean ± SEM of nine independent experiments. The data were analyzed by paired t-test, ** p < 0.01, *** p < 0.001.