Protein Expression Platforms and the Challenges of Viral Antigen Production

Abstract

Several protein expression platforms exist for a wide variety of biopharmaceutical needs. A substantial proportion of research and development into protein expression platforms and their optimization since the mid-1900s is a result of the production of viral antigens for use in subunit vaccine research. This review discusses the seven most popular forms of expression systems used in the past decade-bacterial, insect, mammalian, yeast, algal, plant and cell-free systems-in terms of advantages, uses and limitations for viral antigen production in the context of subunit vaccine research. Post-translational modifications, immunogenicity, efficacy, complexity, scalability and the cost of production are major points discussed. Examples of licenced and experimental vaccines are included along with images which summarize the processes involved.

Keywords: protein expression; subunit vaccines; synthetic biology; viral antigens.

Figure 1

Molecular cloning overview.

Figure 2

Bacterial expression system.

Figure 3

Insect cell (baculovirus) expression system.

Figure 4

Mammalian expression system.

Figure 5

Yeast expression system.

Figure 6

Algal expression system.

Figure 7

Plant expression system.

Figure 8

Cell-free expression system.