Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jan 7;116(1):26.
doi: 10.1007/s00223-024-01335-9.

Adipose-Derived Stromal Cell Conditioned Medium on Bone Remodeling: Insights from a 3D Osteoblast-Osteoclast Co-Culture Model

Elena Della Morte #  1 , Maria Pina Notarangelo #  2 , Stefania Niada  1 , Chiara Giannasi  1   3 , Federica Fortuna  4 , Francesca Cadelano  1   3 , Elisabetta Lambertini  5 , Roberta Piva  2 , Anna Teresa Brini  1   3 , Letizia Penolazzi  6
Affiliations

Adipose-Derived Stromal Cell Conditioned Medium on Bone Remodeling: Insights from a 3D Osteoblast-Osteoclast Co-Culture Model

Elena Della Morte et al. Calcif Tissue Int. .

Abstract

This study describes the potential of the conditioned medium (CM) from adipose-derived mesenchymal stromal cells (ASCs) to affect the response of bone cells and support bone remodeling. This was in particular assessed by an in vitro model represented by a 3D human osteoblast-osteoclast co-culture. It has been reported that the effects of ASCs are predominantly attributable to the paracrine effects of their secreted factors, that are present as soluble factors or loaded into extracellular vesicles. They may affect various biological processes, including bone turnover. Our interest was to provide further evidence to support ASC-CM as a promising cell-free therapeutic agent for the treatment of bone loss. ASC-CM was characterized using nanoparticle tracking analysis (NTA), cytofluorimetry, and proteomic analysis. Human osteoblasts (hOBs) from vertebral lamina were cultured with monocytes, as osteoclasts (hOCs) precursors, in a Rotary cell culture system for 14 days. Histochemical analysis was performed to evaluate the effect of ASC-CM on bone-specific markers such as tartrate-resistant acid phosphatase (TRAP), osteopontin (OPN), RUNX2, Collagen 1 (COL1), and mineral matrix. ASC-CM characterization confirmed the content of CD63/CD81/CD9 positive extracellular vesicles. Proteomic dataset considering bone-remodeling-related keywords identified 16 processes significantly enriched. The exposure of hOBs/hOCs aggregates to ASC-CM induced increase of OPN, COL I, and RUNX2, and significantly induced mineral matrix deposition, while significantly reducing TRAP expression. These data demonstrated that CM from ASCs contains a complex of secreted factors able to control either bone resorption or bone formation and requires further investigations to deeply analyze their potential therapeutic effects.

Keywords: Adipose-derived mesenchymal stromal cells; Bone remodeling; Conditioned medium; Osteoblasts; Osteoclasts.

PubMed Disclaimer

Conflict of interest statement

Declarations. Conflict of interest: Della Morte E, Notarangelo MP, Niada S, Giannasi C, Fortuna F, Cadelano F, Lambertini E, Piva R, Brini AT, and Penolazzi L declare that they have no relevant financial or non-financial interests to disclose. The authors have no competing interests to declare that are relevant to the content of this article. All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest or non-financial interest in the subject matter or materials discussed in this manuscript. The authors have no financial or proprietary interests in any material discussed in this article.

References

    1. Gunawardena TNA, Rahman MT, Abdullah BJJ, Abu Kasim NH (2019) Conditioned media derived from mesenchymal stem cell cultures: the next generation for regenerative medicine. J Tissue Eng Regen Med 13:569–586. https://doi.org/10.1002/term.2806 - DOI - PubMed
    1. Műzes G, Sipos F (2022) Mesenchymal stem cell-derived secretome: a potential therapeutic option for autoimmune and immune-mediated inflammatory diseases. Cells 11:2300. https://doi.org/10.3390/cells11152300 - DOI - PubMed - PMC
    1. Giannasi C, Niada S, Magagnotti C et al (2020) Comparison of two ASC-derived therapeutics in an in vitro OA model: secretome versus extracellular vesicles. Stem Cell Res Ther 11:521. https://doi.org/10.1186/s13287-020-02035-5 - DOI - PubMed - PMC
    1. Chen W, Sun Y, Gu X et al (2019) Conditioned medium of mesenchymal stem cells delays osteoarthritis progression in a rat model by protecting subchondral bone, maintaining matrix homeostasis, and enhancing autophagy. J Tissue Eng Regen Med 13:1618–1628. https://doi.org/10.1002/term.2916 - DOI - PubMed
    1. Liu K, Sakai K, Watanabe J et al (2024) Conditioned medium of human mesenchymal stem cells affects stem cell senescence in osteoporosis. Biochem Biophys Res Commun 711:149858. https://doi.org/10.1016/j.bbrc.2024.149858 - DOI - PubMed

MeSH terms

Substances

LinkOut - more resources