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. 2024 Dec 20:40:e400225.
doi: 10.1590/acb400225. eCollection 2024.

Effect of multiorgan abdominal ischemic preconditioning on experimental kidney transplantation

Juan Cruz Abate  1 , Ivana Ivanoff Marinoff  2 , Nathalie Arnal  3 , Mariana Machuca  4 , Rodrigo Papa-Gobbi  2 , Leandro Vecchio  2 , Martín Rumbo  2 , Pablo Stringa  1   2 , Natalia Raquel Lausada  1
Affiliations

Effect of multiorgan abdominal ischemic preconditioning on experimental kidney transplantation

Juan Cruz Abate et al. Acta Cir Bras. .

Abstract

Purpose: To mitigate ischemia-reperfusion injury (IRI) triggered in solid organ transplant procedures, we aimed to evaluate the effects of multi-organ abdominal ischemic preconditioning (MAIP) in the context of renal IRI.

Methods: An experimental kidney transplant model was conducted. Rats were divided into three groups: an intervention free basal group from which physiological data was collected; a control group (CT), which consisted of transplanted animals without MAIP; and a treated group, in which a MAIP protocol was implemented in the donor during the procurement of the left kidney, monitoring the recipient for 24 hours.

Results: Urea, creatinine, and lactate dehydrogenase, as well as histopathological analysis (Banff: CT 1,66 ± 0,57 vs. basal 0, and MAIP 1), showed a clear trend in favor of MAIP group. Similar results were observed for tumor necrosis factor-α, interleukin-6 and CXCL10, as well as indicators of oxidative stress, with statistically significant levels for CXCL10 [0,295 ± 0,0074 arbitrary units (AU) CT and 0,0057 ± 0,0065 AU MAIP] and TBARS (2,93 ± 0,08 nmol/μg CT; and 2,49 ± 0,23 nmol/μg MAIP; p 0.05).

Conclusion: The findings indicated that the MAIP exerts a protective influence on the transplanted kidneys, functioning as an IRI-protective strategy and enhancing the parameters associated with renal graft functionality.

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Conflict of interest statement

Conflict of interest: Nothing to declare.

Figures

Figure 1
Figure 1. The multiorgan abdominal ischemic preconditioning (MAIP) protocol is executed by clamping the supraceliac abdominal aorta in rats. The primary structures involved include the portal vein, hepatic artery, splenic vein superior mesenteric vein, duodenum, celiac trunk, vena cava, renal artery, abdominal aorta, and superior mesenteric artery.
Figure 2
Figure 2. Results of urea, creatinine, nitrative stress, and histopathological scores in the CT and MAIP animals following prolonged renal ischemia. The most significant differences between the CT group and the treated group were observed in the histological analysis and the levels of nitrosative stress.
Figure 3
Figure 3. Results of urea, creatinine, and lactate dehydrogenase in the basal, CT, and MAIP groups associated with experimental kidney transplantation in rats. The MAIP group demonstrated a statistically significant decrease in urea levels compared to the CT group.
Figure 4
Figure 4. Determinations of mRNA (TNF-α, CXCL10, IL-6) and indicators of oxidative stress, including FRAP, TBARs, and CPs, in the basal, CT, and MAIP groups associated with kidney transplantation.
Figure 5
Figure 5. The Banff score in CT, and MAIP groups associated with kidney transplantation is presented. (a) Representative images of kidneys (hematoxylin and eosin 20x and 40x) demonstrate (b) the absence of lesions, (c) focal tubular necrosis, and (d) hemorrhage as evaluated parameters. Considering all assessed variables, (e) principal component analysis reveals the protective effects of MAIP against renal IRI.
See this image and copyright information in PMC

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