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. 2025 Jan 9;34(1):8.
doi: 10.1007/s11248-024-00420-x.

NtLPA1 overexpression regulates the growth of tobacco and enhances resistance to blight

Guiqin Shi  1 , Yanxiao Bu  2 , Lei Chi  2 , Xifeng Zhang  3 , Yuqing Meng  2 , Shijie Zhang  3 , Geng Tian  4
Affiliations

NtLPA1 overexpression regulates the growth of tobacco and enhances resistance to blight

Guiqin Shi et al. Transgenic Res. .

Abstract

The involvement of Loose Plant Architecture 1 (LPA1) in regulating plant growth and leaf angle has been previously demonstrated. However, the fundamental genetic background remains unidentified. To further understand the tissue expression profile of the NtLPA1 gene, an overexpression vector (pBI121-NtLPA1) was developed and employed to modify tobacco using the leaf disc method genetically. Validation confirmed the generation of transgenic tobacco plants with NtLPA1 overexpression. The findings indicated that increased NtLPA1 overexpression substantially decreased plant auxin sensitivity and modulated signal transduction and polar transport, significantly reducing leaf angle, diminished leaf area during early and late growth stages, and shortened root length. In summary, NtLPA1 augmented tobacco resistance to severe shin disease by modulating the expression of disease-associated genes PBZ1, PR1b, and the growth regulator auxin polar transport factor PIN1.

Keywords: NtLPA1; Leaf angle; Overexpression; Tobacco sore shin.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare that they no conflict of interest.

References

    1. Audran-Delalande C, Bassa C, Mila I, Regad F, Zouine M, Bouzayen M (2012) Genome-wide identification, functional analysis and expression profiling of the Aux/IAA gene family in tomato. Plant Cell Physiol 53:659–672 - PubMed
    1. Chapman EJ, Estelle M (2009) Mechanism of auxin-regulated gene expression in plants. Annu Rev Genet 43:265–285 - PubMed
    1. Chu J, Xu H, Dong H, Xuan YH (2021) Loose plant architecture 1-interacting kinesin-like protein KLP promotes rice resistance to sheath blight disease. Rice 14:60 - PubMed - PMC
    1. Cui D, Zhao J, Jing Y, Fan M, Liu J, Wang Z, Xin W, Hu Y, Yu HJ (2013) The arabidopsis IDD14, IDD15, and IDD16 cooperatively regulate lateral organ morphogenesis and gravitropism by promoting auxin biosynthesis and transport. PLoS Genet 9:e1003759 - PubMed - PMC
    1. Duan K, Li L, Hu P, Xu SP, Xu ZH, Xue HW (2006) A brassinolide-suppressed rice MADS-box transcription factor, OsMDP1, has a negative regulatory role in BR signaling. Plant J 47:519–531 - PubMed

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