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Review
. 2025 Feb;48(2):100178.
doi: 10.1016/j.mocell.2025.100178. Epub 2025 Jan 7.

Essential resources and best practices for laboratory mouse research

Rosa Haque  1 , Aysenur Deniz Song  2 , Jongsun Lee  1 , Seung-Jae V Lee  3 , Jae Myoung Suh  4
Affiliations
Review

Essential resources and best practices for laboratory mouse research

Rosa Haque et al. Mol Cells. 2025 Feb.

Abstract

The laboratory mouse (Mus musculus) is the most widely used mammalian model organism in biomedical and life science research. This concise guide aims to provide essential information to assist researchers new to working with mice, covering topics such as mouse husbandry, maintenance, and available resources for obtaining mouse strains and associated data. Additionally, we discuss ethical considerations, emphasizing the 3Rs (replacement, reduction, and refinement) to ensure responsible and humane research practices.

Keywords: Animal research; Mouse database; Mouse experiments.

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Conflict of interest statement

Declaration of Competing Interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Seung-Jae V. Lee is an Editor-in-Chief for Molecules and Cells and was not involved in the editorial review or the decision to publish this article. ORCID Rosa Haque: https://orcid.org/0009-0001-9292-028X. Aysenur Deniz Song: https://orcid.org/0000-0003-0998-9225. Jongsun Lee: https://orcid.org/0009-0008-9098-6147. Jae Myoung Suh: https://orcid.org/0000-0001-8097-4662. Seung-Jae V. Lee: https://orcid.org/0000-0002-6103-156X.

References

    1. Al-Shaer A.E., Pal A., Shi Q., Carson M.S., Regan J., Behee M., Buddenbaum N., Drawdy C., Davis T., Virk R., et al. Modeling human heterogeneity of obesity with diversity outbred mice reveals a fat mass-dependent therapeutic window for resolvin E1. FASEB J. 2022;36(6) - PMC - PubMed
    1. Arvidsson C., Hallén A., Bäckhed F. Generating and analyzing germ-free mice. Curr. Protoc. Mouse Biol. 2012;2(4):307–316. doi: 10.1002/9780470942390.mo120064. - DOI - PubMed
    1. Beura L.K., Hamilton S.E., Bi K., Schenkel J.M., Odumade O.A., Casey K.A., Thompson E.A., Fraser K.A., Rosato P.C., Filali-Mouhim A., et al. Normalizing the environment recapitulates adult human immune traits in laboratory mice. Nature. 2016;532(7600):512–516. doi: 10.1038/nature17655. - DOI - PMC - PubMed
    1. Bogue M.A., Philip V.M., Walton D.O., Grubb S.C., Dunn M.H., Kolishovski G., Emerson J., Mukherjee G., Stearns T., He H., et al. Mouse Phenome Database: a data repository and analysis suite for curated primary mouse phenotype data. Nucleic Acids Res. 2020;48:D716–D723. - PMC - PubMed
    1. Bryda E.C. The Mighty Mouse: the impact of rodents on advances in biomedical research. Missouri Med. 2013;110(3):207–211. - PMC - PubMed

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