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. 2025 May 5;60(9):1377-1391.e7.
doi: 10.1016/j.devcel.2024.12.025. Epub 2025 Jan 9.

A single-nuclei transcriptome census of the Arabidopsis maturing root identifies that MYB67 controls phellem cell maturation

Charlotte N Miller  1 , Sean Jarrell-Hurtado  1 , Manisha V Haag  1 , Y Sara Ye  1 , Mathew Simenc  1 , Paloma Alvarez-Maldonado  1 , Sara Behnami  1 , Ling Zhang  1 , Joseph Swift  1 , Ashot Papikian  1 , Jingting Yu  2 , Kelly Colt  1 , Joseph R Ecker  3 , Todd P Michael  1 , Julie A Law  4 , Wolfgang Busch  5
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A single-nuclei transcriptome census of the Arabidopsis maturing root identifies that MYB67 controls phellem cell maturation

Charlotte N Miller et al. Dev Cell. .

Abstract

The periderm provides a protective barrier in many seed plant species. The development of the suberized phellem, which forms the outermost layer of this important tissue, has become a trait of interest for enhancing both plant resilience to stresses and plant-mediated CO2 sequestration in soils. Despite its importance, very few genes driving phellem development are known. Employing single-nuclei sequencing, we have generated an expression census capturing the complete developmental progression of Arabidopsis root phellem cells, from their progenitor cell type, the pericycle, through to their maturation. With this, we identify a whole suite of genes underlying this process, including MYB67, which we show has a role in phellem cell maturation. Our expression census and functional discoveries represent a resource, expanding our comprehension of secondary growth in plants. These data can be used to fuel discoveries and engineering efforts relevant to plant resilience and climate change.

Keywords: DAP-seq; development; differentiation; periderm; phellem; roots; snRNA-seq; suberin; transcriptome.

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Conflict of interest statement

Declaration of interests W.B., J.A.L., T.P.M., and J.R.E. are co-founders of Cquesta, a company that works on crop root growth and carbon sequestration.

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