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. 1985 Apr;157(2):550-5.
doi: 10.1016/0014-4827(85)90141-7.

Conversion of dense lysosomes into light lysosomes during hepatocytic autophagy

P O Seglen, A E Solheim

Conversion of dense lysosomes into light lysosomes during hepatocytic autophagy

P O Seglen et al. Exp Cell Res. 1985 Apr.

Abstract

Incubation of isolated rat hepatocytes under conditions which support maximal autophagy (amino acid-free medium) caused a marked alteration in the density distribution of lysosomes in continuous metrizamide gradients (mean peak density reduced from 1.14 to 1.09 g/ml). The autophagic sequestration inhibitor 3-methyladenine (3MA) partially prevented the density shift, presumably by stopping the formation of light autophagosomes which otherwise fuse with dense lysosomes and thereby alter the lysosomal density.

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