Standard: Human gastric organoids

Fan Hong^#¹, Ronghui Tan^#¹, Ting Wang^#^{2

3}, Nanshan Zhong^#⁴, Hongling Zhao³, Rui-Hua Xu^{5

6}, Lin Shen⁷, Yingbin Liu^{8

9}, Xuebiao Yao¹⁰, Dongxi Xiang^{8

9}, Lijian Hui^{11

12}, Jianping Xiong^{13

14}, Dong Gao^{15

16

17}, Bing Zhao⁴, Zhifeng Miao^{18

19

20}, Jie Hao^{21

22}, Yong Li²³, Shijun Hu²⁴, Boqiang Fu²⁵, Guoqiang Hua^{26

27}, Lei Wang^{3

21}, Zhao-Lei Zeng⁵, Chong Chen²⁸, Jianmin Wu^{29

30}, Changlin Wang^{31

32}, Chunnian Wang³³, Xianbao Zhan³⁴, Chen Song³⁵, Chunping Yu³⁶, Yingying Yang³⁷, Gengming Niu³⁸, Yalong Wang³⁹, Tongbiao Zhao^{40

41

42}, Ye-Guang Chen^{43

44

45}

Affiliations

¹ Guangzhou National Laboratory, Guangzhou, 510005, China.
² The State Key Laboratory of Membrane Biology, Tsinghua‑Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
³ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China.
⁴ The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China.
⁵ State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, China.
⁶ Research Unit of Precision Diagnosis and Treatment for Gastrointestinal Cancer, Chinese Academy of Medical Sciences, Guangzhou, 510060, China.
⁷ State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers, Beijing Key Laboratory of Carcinogenesis and Translational Research, Department of Gastrointestinal Oncology, Peking University Cancer Hospital and Institute, Beijing, 100142, China.
⁸ State Key Laboratory of Systems Medicine for Cancer, Shanghai Cancer Institute, Shanghai Jiaotong University School of Medicine, Shanghai, 200232, China.
⁹ Department of Biliary-Pancreatic Surgery, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200127, China.
¹⁰ MOE Key Laboratory of Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, University of Science and Technology of China, Hefei, 230027, China.
¹¹ School of Life Science and Technology, Shanghai Tech University, Shanghai, 201210, China.
¹² State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Science, Shanghai, 200031, China.
¹³ Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330031, Jiangxi, China.
¹⁴ Jiangxi Key Laboratory for Individual Cancer Therapy, Nanchang, 330031, Jiangxi, China.
¹⁵ University of Chinese Academy of Sciences, Beijing, 100049, China.
¹⁶ Key Laboratory of Multi-Cell Systems, Shanghai Key Laboratory of Molecular Andrology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China.
¹⁷ Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen, 518132, China.
¹⁸ Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, 155N Nanjing Street, Shenyang, 110001, Liaoning, China.
¹⁹ Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, China Medical University, Shenyang, 110001, Liaoning, China.
²⁰ Institute of Health Sciences, China Medical University, Shenyang, 110001, Liaoning, China.
²¹ National Stem Cell Resource Center, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
²² State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
²³ Department of Gastrointestinal Surgery, Department of General Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510080, China.
²⁴ Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Suzhou, 510080, China.
²⁵ National Institute of Metrology, Beijing, 100029, China.
²⁶ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200433, China.
²⁷ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China.
²⁸ Gastric Cancer Center and Laboratory of Gastric Cancer, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
²⁹ Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Center for Cancer Bioinformatics, Peking University Cancer Hospital and Institute, Beijing, 100142, China.
³⁰ Peking University International Cancer Institute, Peking University, Beijing, 100191, China.
³¹ China National Institute of Standardization, Beijing, 100191, China.
³² Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China.
³³ Department of Gastrointestinal Pathology, Ningbo Diagnostic Pathology Center, Ningbo, 315021, China.
³⁴ Department of Oncology, Changhai Hospital, Second Military Medical University, No. 168 Changhai Road, Shanghai, 200433, China.
³⁵ Huayi Regeneration Technology Co., Ltd, Chengdu, 611135, China.
³⁶ Lilly (China) Research and Development Center, Shanghai, 201203, China.
³⁷ Qilu Pharmaceutical Co., Ltd, Jinan, 250104, China.
³⁸ Shang Hai OneTar Biomedicine Co., Ltd, Shanghai, 201203, China.
³⁹ Guangzhou National Laboratory, Guangzhou, 510005, China. wangyl16@tsinghua.org.cn.
⁴⁰ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴¹ State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴² Department of Gastrointestinal Surgery, Department of General Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510080, China. tbzhao@ioz.ac.cn.
⁴³ Guangzhou National Laboratory, Guangzhou, 510005, China. ygchen@tsinghua.edu.cn.
⁴⁴ The State Key Laboratory of Membrane Biology, Tsinghua‑Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China. ygchen@tsinghua.edu.cn.
⁴⁵ The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China. ygchen@tsinghua.edu.cn.

^# Contributed equally.

PMID: 39808364
PMCID: PMC11732820
DOI: 10.1186/s13619-024-00218-6

Editorial

Standard: Human gastric organoids

Fan Hong et al. Cell Regen. 2025.

. 2025 Jan 14;14(1):2.

doi: 10.1186/s13619-024-00218-6.

Authors

Affiliations

¹ Guangzhou National Laboratory, Guangzhou, 510005, China.
² The State Key Laboratory of Membrane Biology, Tsinghua‑Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
³ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China.
⁴ The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China.
⁵ State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, China.
⁶ Research Unit of Precision Diagnosis and Treatment for Gastrointestinal Cancer, Chinese Academy of Medical Sciences, Guangzhou, 510060, China.
⁷ State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers, Beijing Key Laboratory of Carcinogenesis and Translational Research, Department of Gastrointestinal Oncology, Peking University Cancer Hospital and Institute, Beijing, 100142, China.
⁸ State Key Laboratory of Systems Medicine for Cancer, Shanghai Cancer Institute, Shanghai Jiaotong University School of Medicine, Shanghai, 200232, China.
⁹ Department of Biliary-Pancreatic Surgery, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200127, China.
¹⁰ MOE Key Laboratory of Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, University of Science and Technology of China, Hefei, 230027, China.
¹¹ School of Life Science and Technology, Shanghai Tech University, Shanghai, 201210, China.
¹² State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Science, Shanghai, 200031, China.
¹³ Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330031, Jiangxi, China.
¹⁴ Jiangxi Key Laboratory for Individual Cancer Therapy, Nanchang, 330031, Jiangxi, China.
¹⁵ University of Chinese Academy of Sciences, Beijing, 100049, China.
¹⁶ Key Laboratory of Multi-Cell Systems, Shanghai Key Laboratory of Molecular Andrology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China.
¹⁷ Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen, 518132, China.
¹⁸ Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, 155N Nanjing Street, Shenyang, 110001, Liaoning, China.
¹⁹ Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, China Medical University, Shenyang, 110001, Liaoning, China.
²⁰ Institute of Health Sciences, China Medical University, Shenyang, 110001, Liaoning, China.
²¹ National Stem Cell Resource Center, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
²² State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
²³ Department of Gastrointestinal Surgery, Department of General Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510080, China.
²⁴ Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Suzhou, 510080, China.
²⁵ National Institute of Metrology, Beijing, 100029, China.
²⁶ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200433, China.
²⁷ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China.
²⁸ Gastric Cancer Center and Laboratory of Gastric Cancer, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
²⁹ Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Center for Cancer Bioinformatics, Peking University Cancer Hospital and Institute, Beijing, 100142, China.
³⁰ Peking University International Cancer Institute, Peking University, Beijing, 100191, China.
³¹ China National Institute of Standardization, Beijing, 100191, China.
³² Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China.
³³ Department of Gastrointestinal Pathology, Ningbo Diagnostic Pathology Center, Ningbo, 315021, China.
³⁴ Department of Oncology, Changhai Hospital, Second Military Medical University, No. 168 Changhai Road, Shanghai, 200433, China.
³⁵ Huayi Regeneration Technology Co., Ltd, Chengdu, 611135, China.
³⁶ Lilly (China) Research and Development Center, Shanghai, 201203, China.
³⁷ Qilu Pharmaceutical Co., Ltd, Jinan, 250104, China.
³⁸ Shang Hai OneTar Biomedicine Co., Ltd, Shanghai, 201203, China.
³⁹ Guangzhou National Laboratory, Guangzhou, 510005, China. wangyl16@tsinghua.org.cn.
⁴⁰ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴¹ State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
⁴² Department of Gastrointestinal Surgery, Department of General Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510080, China. tbzhao@ioz.ac.cn.
⁴³ Guangzhou National Laboratory, Guangzhou, 510005, China. ygchen@tsinghua.edu.cn.
⁴⁴ The State Key Laboratory of Membrane Biology, Tsinghua‑Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China. ygchen@tsinghua.edu.cn.
⁴⁵ The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China. ygchen@tsinghua.edu.cn.

^# Contributed equally.

PMID: 39808364
PMCID: PMC11732820
DOI: 10.1186/s13619-024-00218-6

Abstract

Organoid technology provides a transformative approach to understand human physiology and pathology, offering valuable insights for scientific research and therapeutic development. Human gastric organoids, in particular, have gained significant interest for applications in disease modeling, drug discovery, and studies of tissue regeneration and homeostasis. However, the lack of standardized quality control has limited their extensive clinical applications. The "Human Gastric Organoids" is part of a series of guidelines for human gastric organoids in China, which establishes comprehensive standards on terminology, technical specifications, testing methods, inspection rules, usage instructions, labeling, transportation, and storage, developed by experts from the Chinese Society for Cell Biology and its branch societies. Released on October 29, 2024, this guideline aims to establish standardized protocols, enhance institutional practices, and promote international standardization for clinical and research applications of human gastric organoids.

Keywords: Definitions; Gastric organoids; Human stomach; Labeling; Standard; Storage; Technical requirements; Terms; Transportation.

PubMed Disclaimer

Conflict of interest statement

Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: Y.-G.C. is the Editor‑in‑Chief of Cell Regeneration. He was not involved in the review or decision related to this manuscript. This work was not sponsored by any commercial organizations, and all the other authors declare that they have no competing interests.

References

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Standard: Human gastric organoids

Affiliations

Standard: Human gastric organoids

Authors

Affiliations

Abstract

Conflict of interest statement

References

Publication types

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