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. 2025 Jan 14;80(1):36.
doi: 10.1007/s11130-024-01266-8.

Extraction and Characterization of Inulin-Like Fructans from Hydroponically Grown Stevia rebaudiana Roots for Food Applications

Hevelyn Regina da Silva Lima  1 , Gabriela Lafayne Okonski Dos Santos  2 , Susana Tavares Cotrim Ribeiro  2 , Maria Amélia Gonçalves  2 , Aline Savam  2 , Regina Aparecida Correia Gonçalves  2 , Arildo José Braz de Oliveira  3   4
Affiliations

Extraction and Characterization of Inulin-Like Fructans from Hydroponically Grown Stevia rebaudiana Roots for Food Applications

Hevelyn Regina da Silva Lima et al. Plant Foods Hum Nutr. .

Abstract

Stevia rebaudiana is a plant native to South America known for producing steviol glycosides and fructans used in low-calorie and functional foods. This study aimed to cultivate and isolate inulin from hydroponically grown S. rebaudiana roots. Seedlings were grown hydroponically for 28 days; then, roots were dried and extracted at 121 °C for 20 min. Extracts were centrifuged and lyophilized, yielding supernatant (SSR-H) and precipitate (PSR-H) fractions. Fructan yields were 21.34% (SSR-H) and 2.91% (PSR-H). HPLC, FTIR, and NMR analyses confirmed inulin-like fructans with polymerization degrees of 16 and 31, respectively. The characteristic peaks of a fructan-like-inulin were observed in the FTIR, ¹H, and ¹³C NMR spectra. These results demonstrate effective inulin extraction from hydroponically grown S. rebaudiana roots, highlighting its potential applications in the food industry.

Keywords: Stevia rebaudiana; Autoclave extraction; Fructan; Hydroponic culture.

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Conflict of interest statement

Declarations. Ethical Approval: This article does not contain any studies with human or animal subjects. Competing Interests: The authors declare no competing interests.

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