Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Nov 21:35:e2404029.
doi: 10.4014/jmb.2409.04029.

Cholesterol-Lowering Activity of Lactiplantibacillus pentosus KS6I1 in High-Cholesterol Diet-Induced Hypercholesterolemic Mice

Karthiyaini Damodharan  1 , Sasikumar Arunachalam Palaniyandi  2 , Seung Hwan Yang  3 , Seetharaman Balaji  4
Affiliations

Cholesterol-Lowering Activity of Lactiplantibacillus pentosus KS6I1 in High-Cholesterol Diet-Induced Hypercholesterolemic Mice

Karthiyaini Damodharan et al. J Microbiol Biotechnol. .

Abstract

Hypercholesterolemia is a risk factor of coronary heart disease and cholesterol-lowering probiotics are seen as alternative to drugs for the management of this condition. In the present study, we evaluated the cholesterol-lowering activity of Lactiplantibacillus pentosus KS6I1 in high-cholesterol diet-induced hypercholesterolemic mice. The mice were fed with high-cholesterol diet (HCD) and were divided into three groups: HCD group, KS6I1 group (fed with HCD + 200 μl of 1010 CFU/ml L. pentosus KS6I1), and L.ac group (fed with HCD + 200 μl of 1010 CFU/ml L. acidophilus ATCC 43121 as the positive control). Simultaneously, a normal control diet (NCD) group was maintained. After 6 weeks, the low-density lipoprotein (LDL)-cholesterol and total cholesterol levels were significantly reduced in the blood plasma of KS6I1 group mice. Analysis of liver tissue showed a decrease in total cholesterol and LDL-cholesterol and increase in triglyceride levels in KS6I1 compared to those in HCD group. Fecal total cholesterol and total bile acid content was significantly increased in the KS6I1 group than in other groups. Additionally, gene expression analysis showed that LDLR, SREBF2, CYP7A1 genes were significantly upregulated in KS6I1 group compared to the HCD group, while the expression of NPC1L1 gene was significantly reduced in KS6I1 group compared to HCD group. These observations show that the cholesterol-lowering effect of L. pentosus KS6I1 could be attributed to increased excretion of bile acids and cholesterol in the feces of mice. These results indicate that L. pentosus KS6I1 could be developed into a potential probiotic for hypercholesterolemia.

Keywords: Lactiplantibacillus pentosus; Probiotics; bile salt deconjugation; cholesterol-lowering activity.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest

The authors have no financial conflicts of interest to declare.

Figures

Fig. 1
Fig. 1. Bodyweight (A) and food intake (B) of ICR mice receiving various treatments.
(■) Normal, (□) HCD, (●) L.ac, (Δ) KS6I1. The results are presented as mean ± SD (n = 6/group).
Fig. 2
Fig. 2. Effect of L. pentosus strain KS6I1 on plasma biochemical parameters such as total cholesterol (A) LDL cholesterol (B) HDL-cholesterol (C) and triglyceride (D) in mice after 6 weeks.
Bars with different letters indicate significantly different from each other and bars sharing letters are not significantly different.
Fig. 3
Fig. 3. Effect of L. pentosus strain KS6I1 on liver biochemical parameters such as total cholesterol (A) LDL cholesterol (B) HDL-cholesterol (C) and triglyceride (D) in mice after 6 weeks.
Bars with different letters indicate significantly different from each other and bars sharing letters are not significantly different.
Fig. 4
Fig. 4. Effect of L. pentosus strain KS6I1 on fecal biochemical parameters such as total cholesterol (A) LDL cholesterol (B) HDL-cholesterol (C) triglyceride (D) and total bile acids (E) in mice after 6 weeks.
Bars with different letters indicate significantly different from each other and bars sharing letters are not significantly different.
Fig. 5
Fig. 5. Effects of KS6I1 feeding on expression levels of cholesterol metabolism-related genes (LDL receptor, SREBP-2, and CYP7A1) in mice liver and NPC1L1 in mice intestine.
Data were normalized to β-actin RNA expression levels and then compared to the HCD group. *, p < 0.05, **, p < 0.005, ***, p < 0.001, vs HCD group.
See this image and copyright information in PMC

References

    1. Zheng J, Wittouck S, Salvetti E, Franz C, Harris HMB, Mattarelli P, et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. Int. J. Syst. Evol. Microbiol. 2020;70:2782–2858. doi: 10.1099/ijsem.0.004107. - DOI - PubMed
    1. Jamyuang C, Phoonlapdacha P, Chongviriyaphan N, Chanput W, Nitisinprasert S, Nakphaichit M. Characterization and probiotic properties of Lactobacilli from human breast milk. 3 Biotech. 2019;9:398. doi: 10.1007/s13205-019-1926-y. - DOI - PMC - PubMed
    1. Kangwan N, Kongkarnka S, Boonkerd N, Unban K, Shetty K, Khanongnuch C. Protective effect of probiotics isolated from traditional fermented tea leaves (Miang) from Northern Thailand and role of synbiotics in ameliorating experimental ulcerative colitis in mice. Nutrients. 2022;14:227. doi: 10.3390/nu14010227. - DOI - PMC - PubMed
    1. Ma Y, Hu C, Yan W, Jiang H, Liu G. Lactobacillus pentosus increases the abundance of Akkermansia and affects the serum metabolome to alleviate DSS-induced colitis in a murine model. Front. Cell Dev. Biol. 2020;8:591408. doi: 10.3389/fcell.2020.591408. - DOI - PMC - PubMed
    1. Gu M, Werlinger P, Cho JH, Jang N, Choi SS, Suh JW, et al. Lactobacillus pentosus MJM60383 inhibits lipid accumulation in Caenorhabditis elegans induced by Enterobacter cloacae and glucose. Int. J. Mol. Sci. 2022;24:280. doi: 10.3390/ijms24010280. - DOI - PMC - PubMed

MeSH terms

LinkOut - more resources