Molecular advances in research and applications of male sterility systems in tomato

Abstract

Tomato, belonging to the nightshade family, is globally considered as a model system for classical and molecular genetics, genomics, and reproductive developmental studies. In the current scenario of climate change, hybrid development is among the crucial elements in the genetic improvement of crop plants. The phenomenon of male sterility is a viable approach for ensuring hybrid seed purity and reducing the cost of hybrid seed production. This review aims to shed light on the use of neoteric genomics and genome editing tools in understanding the genetics and molecular regulation of male sterility in tomato. Plant male gametophyte development is highly susceptible to environmental stress. Abnormalities at any stage of male reproductive development, such as premature or delayed tapetal cell degradation triggered by oxidative stress and programmed cell death (PCD) leads to male sterility in tomato. In tomato, more than 55 sporogenous, structural, and functional male sterile mutants, which are mainly under the control of recessive nuclear genes, have been reported. Recently, the role of open reading frames (ORFs) in governing cytoplasmic male sterility in tomato has also been documented. This review highlights the genetic and genomic progress in the investigation of underlying molecular pathways and practical application of potential male sterile mutants in tomato breeding. The applications and future prospects of genome engineering with CRISPR/Cas9 and mitoTALEN in the generation of novel male sterile systems to expedite tomato breeding is discussed.

Keywords: CRISPR/Cas9; Genomics; Hybrid breeding; Male sterility; Plant reproduction; Tomato.