Recommendations for mitochondria transfer and transplantation nomenclature and characterization

Jonathan R Brestoff¹, Keshav K Singh², Katia Aquilano³, Lance B Becker^{4

5}, Michael V Berridge⁶, Eric Boilard⁷, Andrés Caicedo^{8

9}, Clair Crewe^{10

11}, José Antonio Enríquez^{12

13}, Jianqing Gao^{14

15

16}, Åsa B Gustafsson¹⁷, Kazuhide Hayakawa¹⁸, Maroun Khoury^{19

20}, Yun-Sil Lee²¹, Daniele Lettieri-Barbato³, Patricia Luz-Crawford^{19

20}, Heidi M McBride²², James D McCully²³, Ritsuko Nakai²⁴, Jiri Neuzil^{25

26

27}, Martin Picard^{28

29

30

31}, Alexander G Rabchevsky³², Anne-Marie Rodriguez³³, Shiladitya Sengupta³⁴, Alexander J Sercel³⁵, Toshio Suda³⁶, Michael A Teitell³⁷, Alain R Thierry³⁸, Rong Tian³⁹, Melanie Walker⁴⁰, Minghao Zheng^{41

42}

Affiliations

¹ Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA. brestoff@wustl.edu.
² Department of Genetics, I Heersink School of Medicine, University of Alabama at Birmhingham, Birmingham, AL, USA. kksingh@uab.edu.
³ Department of Biology, University of Rome Tor Vergata, Rome, Italy.
⁴ Department of Emergency Medicine, Northwell Health, Manhassett, NY, USA.
⁵ Department of Emergency Medicine, Kindai University Faculty of Medicine, Osaka, Japan.
⁶ Department of Cancer Cell Biology, Malaghan Institute of Medical Research, Wellington, New Zealand.
⁷ Département de Microbiologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec - Université Laval, Québec, Québec, Canada.
⁸ Instituto de Investigaciones en Biomedicina and Colegio de Ciencias de la Salud, Escuela de Medicina, Universidad San Francisco de Quito, Quito, Ecuador.
⁹ Mito-Act Research Consortium, Quito, Ecuador.
¹⁰ Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA.
¹¹ Division of Endocrinology, Metabolism and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, USA.
¹² Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.
¹³ Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable, Instituto de salud Carlos III (CIBERFES), Madrid, Spain.
¹⁴ College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
¹⁵ State Key Laboratory of Advanced Drug Delivery and Release Systems, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
¹⁶ Department of Pharmacy, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
¹⁷ Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA, USA.
¹⁸ Neuroprotection Research Laboratories, Harvard Medical School, Massachusetts General Hospital East 149-2401, Charlestown, MA, USA.
¹⁹ IMPACT Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Universidad de los Andes, Santiago, Chile.
²⁰ Centro de Investigación Biomédica, Facultad de Medicina, Universidad de Los Andes, Santiago, Chile.
²¹ Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
²² Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
²³ Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
²⁴ Department of Hematology and Oncology, Graduate School of Medicine, Osaka University, Osaka, Japan.
²⁵ School of Pharmacy and Medical Science, Griffith University, Southport, Queensland, Australia.
²⁶ Institute of Biotechnology, Czech Academy of Sciences, Prague, Czech Republic.
²⁷ Faculty of Science and First Faculty of Medicine, Charles University, Prague, Czech Republic.
²⁸ Department of Psychiatry, Division of Behavioral Medicine, Columbia University Irving Medical Center, New York, NY, USA.
²⁹ Department of Neurology, H. Houston Merritt Center for Neuromuscular and Mitochondrial Disorders, Columbia University Irving Medical Center, New York, NY, USA.
³⁰ New York State Psychiatric Institute, New York, NY, USA.
³¹ Robert N Butler Columbia Aging Center, Columbia University Mailman School of Public Health, New York, NY, USA.
³² Department of Physiology & the Spinal Cord & Brain Injury Research Center, University of Kentucky, Lexington, KY, USA.
³³ UMR CNRS 8263, INSERM U1345, Development, Adaptation and Ageing, Sorbonne Université, Institut de Biologie Paris-Seine (IBPS), Paris, France.
³⁴ Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.
³⁵ MitoWorld, National Laboratory for Education Transformation, Oakland, CA, USA.
³⁶ Institute of Hematology, Blood Diseases Hospital, Chinese Academy of Sciences and Peking Union Medical College, Tianjin, China.
³⁷ Department of Pathology and Laboratory Medicine, Department of Bioengineering, and Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA, USA.
³⁸ Institute of Research in Cancerology of Montpellier, INSERM U1194, University of Montpellier, ICM, Institut du Cancer de Montpellier, Montpellier, France.
³⁹ Mitochondria and Metabolism Center, Department of Anesthesiology & Pain Medicine, University of Washington, Seattle, WA, USA.
⁴⁰ Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA, USA.
⁴¹ Centre for Orthopaedic Research, Medical School of the University of Western Australia, Nedlands, Western Australia, Australia.
⁴² Perron Institute for Neurological and Translational Science, Nedlands, Western Australia, Australia.

PMID: 39820558
DOI: 10.1038/s42255-024-01200-x

Review

Recommendations for mitochondria transfer and transplantation nomenclature and characterization

Jonathan R Brestoff et al. Nat Metab. 2025 Jan.

. 2025 Jan;7(1):53-67.

doi: 10.1038/s42255-024-01200-x. Epub 2025 Jan 16.

Authors

Affiliations

¹ Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA. brestoff@wustl.edu.
² Department of Genetics, I Heersink School of Medicine, University of Alabama at Birmhingham, Birmingham, AL, USA. kksingh@uab.edu.
³ Department of Biology, University of Rome Tor Vergata, Rome, Italy.
⁴ Department of Emergency Medicine, Northwell Health, Manhassett, NY, USA.
⁵ Department of Emergency Medicine, Kindai University Faculty of Medicine, Osaka, Japan.
⁶ Department of Cancer Cell Biology, Malaghan Institute of Medical Research, Wellington, New Zealand.
⁷ Département de Microbiologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec - Université Laval, Québec, Québec, Canada.
⁸ Instituto de Investigaciones en Biomedicina and Colegio de Ciencias de la Salud, Escuela de Medicina, Universidad San Francisco de Quito, Quito, Ecuador.
⁹ Mito-Act Research Consortium, Quito, Ecuador.
¹⁰ Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA.
¹¹ Division of Endocrinology, Metabolism and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, USA.
¹² Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.
¹³ Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable, Instituto de salud Carlos III (CIBERFES), Madrid, Spain.
¹⁴ College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
¹⁵ State Key Laboratory of Advanced Drug Delivery and Release Systems, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
¹⁶ Department of Pharmacy, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
¹⁷ Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA, USA.
¹⁸ Neuroprotection Research Laboratories, Harvard Medical School, Massachusetts General Hospital East 149-2401, Charlestown, MA, USA.
¹⁹ IMPACT Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Universidad de los Andes, Santiago, Chile.
²⁰ Centro de Investigación Biomédica, Facultad de Medicina, Universidad de Los Andes, Santiago, Chile.
²¹ Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
²² Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
²³ Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
²⁴ Department of Hematology and Oncology, Graduate School of Medicine, Osaka University, Osaka, Japan.
²⁵ School of Pharmacy and Medical Science, Griffith University, Southport, Queensland, Australia.
²⁶ Institute of Biotechnology, Czech Academy of Sciences, Prague, Czech Republic.
²⁷ Faculty of Science and First Faculty of Medicine, Charles University, Prague, Czech Republic.
²⁸ Department of Psychiatry, Division of Behavioral Medicine, Columbia University Irving Medical Center, New York, NY, USA.
²⁹ Department of Neurology, H. Houston Merritt Center for Neuromuscular and Mitochondrial Disorders, Columbia University Irving Medical Center, New York, NY, USA.
³⁰ New York State Psychiatric Institute, New York, NY, USA.
³¹ Robert N Butler Columbia Aging Center, Columbia University Mailman School of Public Health, New York, NY, USA.
³² Department of Physiology & the Spinal Cord & Brain Injury Research Center, University of Kentucky, Lexington, KY, USA.
³³ UMR CNRS 8263, INSERM U1345, Development, Adaptation and Ageing, Sorbonne Université, Institut de Biologie Paris-Seine (IBPS), Paris, France.
³⁴ Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.
³⁵ MitoWorld, National Laboratory for Education Transformation, Oakland, CA, USA.
³⁶ Institute of Hematology, Blood Diseases Hospital, Chinese Academy of Sciences and Peking Union Medical College, Tianjin, China.
³⁷ Department of Pathology and Laboratory Medicine, Department of Bioengineering, and Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA, USA.
³⁸ Institute of Research in Cancerology of Montpellier, INSERM U1194, University of Montpellier, ICM, Institut du Cancer de Montpellier, Montpellier, France.
³⁹ Mitochondria and Metabolism Center, Department of Anesthesiology & Pain Medicine, University of Washington, Seattle, WA, USA.
⁴⁰ Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA, USA.
⁴¹ Centre for Orthopaedic Research, Medical School of the University of Western Australia, Nedlands, Western Australia, Australia.
⁴² Perron Institute for Neurological and Translational Science, Nedlands, Western Australia, Australia.

PMID: 39820558
DOI: 10.1038/s42255-024-01200-x

Abstract

Intercellular mitochondria transfer is an evolutionarily conserved process in which one cell delivers some of their mitochondria to another cell in the absence of cell division. This process has diverse functions depending on the cell types involved and physiological or disease context. Although mitochondria transfer was first shown to provide metabolic support to acceptor cells, recent studies have revealed diverse functions of mitochondria transfer, including, but not limited to, the maintenance of mitochondria quality of the donor cell and the regulation of tissue homeostasis and remodelling. Many mitochondria-transfer mechanisms have been described using a variety of names, generating confusion about mitochondria transfer biology. Furthermore, several therapeutic approaches involving mitochondria-transfer biology have emerged, including mitochondria transplantation and cellular engineering using isolated mitochondria. In this Consensus Statement, we define relevant terminology and propose a nomenclature framework to describe mitochondria transfer and transplantation as a foundation for further development by the community as this dynamic field of research continues to evolve.

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Conflict of interest statement

Competing interests: J.R.B. is a member of the Scientific Advisory Board of LUCA Science, Inc.; receives research support from LUCA Science and Edgewise Therapeutics; is a consultant for Columbus Instruments, Inc.; has consulted for DeciBio within the past 12 months; receives royalties from Springer Nature Group; is an inventor on technology licensed to Columbus Instruments, Inc. with royalty rights; is an inventor on pending patent applications related to the treatment of metabolic diseases (63/625,555) and allergic diseases (US20210128689A1) and mitochondria transfer (018984/US); and is a co-founder and holds equity in Symbiogenix, Inc. K.K.S. is a co-founder, holds equity in, and serves on the Scientific Advisory Board for YUVA Biosciences. E.B. holds patents on detection of extracellular mitochondria in inflammatory conditions (WO2015051466A1) and on mitochondrial autoantibodies in autoimmune diseases (WO2022/246565A1), and is a principal investigator and scientific advisor to MitrixBio. L.B. receives grant support from Philips Medical Systems, ZOLL Medical Corp, Nihon Kohden, PCORI, BrainCool and United Therapeutics; is on the Scientific Advisory Board for Nihon Kohden, HP and Philips; holds seven issued patents and several pending patent applications involving the use of medical slurries as human coolant devices, the creation of slurries, reperfusion cocktails, and measurement of respiratory quotient; and serves on committees for the American Heart Association, which has a financial interest in the outcome of resuscitation studies being conducted and that sells training materials worldwide on resuscitation techniques. A.C. is a founder of and scientific advisor to Dragon Biomed and a scientific advisor to Luvigix. J.A.E. has collaborated with Minova Therapeutics. Å.B.G. is a consultant for Lexeo Therapeutics. J.D.M. has pending patents for the isolation and use of isolated mitochondria and is a founder and member of the Scientific Advisory Board and Board of Directors for cellvie. M.K. is the chief scientific officer of Cells for Cells, EVast Bio, and Regenero (Chilean consortium for regenerative medicine) with Corporación de Fomento de la Producción support; has received research support from ANID (National Agency for Research and Development) basal FB210024 and Cells for Cells; is an inventor on patents related to mesenchymal stem cells (pending patents WO2014135924A1, WO20170646770A2, WO2017064672A1 and WO2019051623). R.N. receives research support from LUCA Science, Inc. A.J.S. is an employee of MitoWorld. M.A.T. is a co-founder and shareholder for NanoCav, a private start-up company with licences for mitochondria-transfer techniques and applications. A.R.T. is an inventor of a pending patent related to the diagnostic detection of cf-mtDNA (WO 2016/063122 A1). R.T. is a scientific advisor for Cytokinetics, Inc. and GenKardia, Inc. M.W. has a pending patent (US20210085713A1) related to compositions and methods for treating stroke. The other authors declare no competing interests.

References

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Recommendations for mitochondria transfer and transplantation nomenclature and characterization

Affiliations

Recommendations for mitochondria transfer and transplantation nomenclature and characterization

Authors

Affiliations

Abstract

Conflict of interest statement

References

Publication types

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources