Nerve growth factor. A structural relationship between its proteolytic and leukocyte-chemotactic active sites
- PMID: 3982403
- DOI: 10.1007/BF00231825
Nerve growth factor. A structural relationship between its proteolytic and leukocyte-chemotactic active sites
Abstract
High molecular weight mouse nerve growth factor (HMW-NGF), in addition to its effects on certain neural elements, is also chemotactic for human polymorphonuclear leukocytes. One of the subunits of HMW-NGF is a protease of the serine family and its active site contains a serine residue and a closely-neighboring histidine residue that are both essential for proteolysis. Elimination of enzyme activity by irreversibly blocking the single serine has no effect on leukotaxis, but blocking the histidine abolishes leukotaxis. These results suggest the possibility that part of the proteolytic active site of this enzyme may have evolved to perform more than one, completely different, biologic function-proteolysis as well as nonproteolytically mediated chemotaxis.
Similar articles
-
Nerve growth factor: stimulation of polymorphonuclear leukocyte chemotaxis in vitro.Proc Natl Acad Sci U S A. 1983 Dec;80(23):7215-8. doi: 10.1073/pnas.80.23.7215. Proc Natl Acad Sci U S A. 1983. PMID: 6580641 Free PMC article.
-
Nerve growth factor: a chemotactic factor for polymorphonuclear leukocytes in vivo.J Immunol. 1985 Jan;134(1):564-8. J Immunol. 1985. PMID: 3964821
-
Nerve growth factor: biosynthetic products of the mouse salivary glands. Characterization of stable high molecular weight and 32,000-dalton nerve growth factors.Biochemistry. 1986 Sep 23;25(19):5565-71. doi: 10.1021/bi00367a033. Biochemistry. 1986. PMID: 3778873
-
Pharmacologic manipulation of leukocyte chemotaxis. Present knowledge and future trends.Am J Med. 1983 Oct 31;75(4B):10-8. doi: 10.1016/0002-9343(83)90323-6. Am J Med. 1983. PMID: 6314812 Review.
-
Measurement of chemotaxis of polymorphonuclear leukocytes in vitro. The problems of the control of gradients of chemotactic factors, of the control of the cells and of the separation of chemotaxis from chemokinesis.J Immunol Methods. 1988 Apr 6;108(1-2):1-18. doi: 10.1016/0022-1759(88)90396-1. J Immunol Methods. 1988. PMID: 3280683 Review. No abstract available.