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. 1985;17(2):90-5.
doi: 10.1159/000265356.

In vivo assessment of lens transmission for blue-green light by autofluorescence measurement

J A van Best, E W Tsoi, J P Boot, J A Oosterhuis

In vivo assessment of lens transmission for blue-green light by autofluorescence measurement

J A van Best et al. Ophthalmic Res. 1985.

Abstract

A simple quantitative and reproducible method for evaluating lens transmission (lambda = 490 and 530 nm) is described. It is based on the measurement of autofluorescence in the anterior and the posterior part of the lens by means of a fluorophotometer, assuming an about equal fluorescence quantum efficiency in both parts. Consequently any difference in fluorescence between both parts can be attributed to a loss of exciting and fluorescent light in the lens. For both wavelengths, the average lens transmission is determined by this method in a normal population as a function of age.

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