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. 2025 May;22(5):e202402087.
doi: 10.1002/cbdv.202402087. Epub 2025 Jan 20.

Exploring the Anti-Adherence Potential of Skt35 to Combat Catheter-Associated Staphylococcus aureus Infections: Efficacy, Toxicity and Mechanism of Action

Ramu Meenatchi  1 , Snega Priya  1 , Chakraborty Shreya  1 , Sanjay Gopi  1 , Rajakrishnan Rajagopal  2 , S Kaliraj  3 , Kathiravan Muthu Kumaradoss  4 , Jesu Arockiaraj  1
Affiliations

Exploring the Anti-Adherence Potential of Skt35 to Combat Catheter-Associated Staphylococcus aureus Infections: Efficacy, Toxicity and Mechanism of Action

Ramu Meenatchi et al. Chem Biodivers. 2025 May.

Abstract

Catheter-associated urinary tract infections (CAUTIs), often caused by biofilm-forming Staphylococcus aureus, present significant clinical challenges. Skt35, a dioxopiperidinamide derivative of cinnamic acid, was investigated for its potential antibacterial and antibiofilm activities against S. aureus biofilms. The antibacterial effect of Skt35 was assessed using the zone of inhibition and microdilution methods, revealing a minimum inhibitory concentration (MIC) of 250 µM. Antibiofilm properties were confirmed through crystal violet assays, scanning electron microscopy and confocal laser scanning microscopy, showing significant biofilm inhibition at the Sub-MIC. In an in vitro bladder model, Skt35-coated silicone catheter tubes exhibited significant antiadhesive effects. Zebrafish embryo tests indicated no toxicity at concentrations up to 125 µM. Molecular docking and simulation analysis revealed strong binding affinities of Skt35 to Accessory Gene Regulator A (-7.9 kcal/mol) and Lux Small protein (-4.96 kcal/mol), suggesting potential disruption of quorum sensing and gene expression in S. aureus, making it a promising candidate for catheter coatings to prevent CAUTIs.

Keywords: Staphylococcus aureus; antibacterial; antibiofilm; catheter‐associated urinary tract infections (CAUTI); dioxopiperidinamide.

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References

    1. S. Boddu, R. Karthikeyan, and K. Mandava, “A Comprehensive Review of Urinary Tract Infections: Pathophysiology, Antibiotic Resistance, and Therapeutic Strategies, With Emphasis on Herbal Alternatives,” Reviews and Research in Medical Microbiology. Published ahead of print, April 16, 2024, https://doi.org/10.1097/MRM.0000000000000400.
    1. A. Flores‐Mireles, T. N. Hreha, and D. A. Hunstad, “Pathophysiology, Treatment, and Prevention of Catheter‐Associated Urinary Tract Infection,” Topics in Spinal Cord Injury Rehabilitation 25 (2019): 228–240.
    1. M. J. Andersen and A. L. Flores‐Mireles, “Urinary Catheter Coating Modifications: The Race Against Catheter‐Associated Infections,” Coatings 10 (2019): 23.
    1. J. J. Molina, K. N. Kohler, C. Gager, et al., “Fibrinolytic‐Deficiencies Predispose Hosts to Septicemia From a Catheter‐Associated UTI,” Nature Communications 15 (2024): 2704.
    1. L. E. Nicolle, “Catheter Associated Urinary Tract Infections,” Antimicrobial Resistance and Infection Control 3 (2014): 23.

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