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Comparative Study
. 2025;21(5):573-586.
doi: 10.2174/0115734099281920240730051328.

Comparative Study on Sedative and Hypnotic Effects of Crude and Parched Semen Ziziphi Spinosae: Integration of Network Pharmacology and In Vivo Pharmacological Evaluation

Jing Xia  1 , Ming Cai  1 , Bo Xu  1 , Guang-Jing Xie  1 , Ping Wang  1
Affiliations
Comparative Study

Comparative Study on Sedative and Hypnotic Effects of Crude and Parched Semen Ziziphi Spinosae: Integration of Network Pharmacology and In Vivo Pharmacological Evaluation

Jing Xia et al. Curr Comput Aided Drug Des. 2025.

Abstract

Objectives: This study aimed to investigate the medicinal properties of SZS before and after processing and provide novel insights into its potential for treating insomnia.

Methods: This study employed the network pharmacology platform to gather information on the chemical composition of SZS, human targets, genes, molecular networks, and pathways associated with insomnia treatment using SZS. Liquid chromatography-tandem mass spectrometry (LC-MS/ MS) was utilized to analyze the chemical profiles of crude SZS, parched SZS, and their combined decoction. The effects of different SZS products on p-chlorophenylalanine-induced insomnia mice were evaluated through pentobarbital-induced sleep tests, behavioral analyses, examination of brain tissue-related mRNA levels, and measurement of plasma neurotransmitters, aiming to explore the sedative and hypnotic effects of various SZS products.

Results: SZS was found to contain a total of 47 genes, including 22 target genes associated with insomnia. These genes may contribute to the sedative and hypnotic effects through 9 related pathways and 69 biological processes. The active components of SZS remained consistent before and after processing. Jujuboside B was found in higher concentrations in crude SZS, while jujuboside A was more abundant in parched SZS. Additionally, SZS exhibited reduced locomotor activity in mice, enhanced the hypnotic effect of pentobarbital sodium, and decreased the levels of acetylcholinesterase, α-1B adrenergic receptor, and solute carrier family 6 member 4 mRNA in the cortex and hippocampus of mice. The levels of acetylcholine, choline acetyltransferase, 5-hydroxyindoleacetic acid, and glutamate in plasma increased, with the hypnotic effect being proportional to the dosage of the drug.

Conclusion: SZS demonstrates sedative and hypnotic effects, potentially mediated by its influence on neurotransmitter levels and related receptors within the central nervous system. There was a slight variation in regulatory capabilities before and after SZS processing, with the combined decoction of crude and parched SZS exhibiting a more pronounced effect, particularly at higher dosages.

Keywords: Semen Ziziphi Spinosae; hypnosis.; insomnia; network pharmacology; processing; sedation.

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References

    1. Roth T.; Krystal A.D.; Lieberman J.A.; Long-term issues in the treatment of sleep disorders. CNS Spectr 2007,12(7)(Suppl. 10),1-14 - PubMed
    1. Bhaskar S.; Hemavathy D.; Prasad S.; Prevalence of chronic insomnia in adult patients and its correlation with medical comorbidities. J Family Med Prim Care 2016,5(4),780-784 - DOI - PubMed
    1. Sivertsen B.; Lallukka T.; Salo P.; Pallesen S.; Hysing M.; Krokstad S.; Øverland S.; Insomnia as a risk factor for ill health: Results from the large population-based prospective Study in orway. J Sleep Res 2014,23(2),124-132 - DOI - PubMed
    1. Asnis G.; Thomas M.; Henderson M.; Pharmacotherapy treatment options for insomnia: A primer for clinicians. Int J Mol Sci 2015,17(1),50 - DOI - PubMed
    1. Wilt T.J.; MacDonald R.; Brasure M.; Olson C.M.; Carlyle M.; Fuchs E.; Khawaja I.S.; Diem S.; Koffel E.; Ouellette J.; Butler M.; Kane R.L.; Pharmacologic treatment of insomnia disorder: An evidence report for a clinical practice guideline by the american college of physicians. Ann Intern Med 2016,165(2),103-112 - DOI - PubMed

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