New Species of Diaporthales (Ascomycota) from Diseased Leaves in Fujian Province, China

Abstract

Fungal biota represents important constituents of phyllosphere microorganisms. It is taxonomically highly diverse and influences plant physiology, metabolism and health. Members of the order Diaporthales are distributed worldwide and include devastating plant pathogens as well as endophytes and saprophytes. However, many phyllosphere Diaporthales species remain uncharacterized, with studies examining their diversity needed. Here, we report on the identification of several diaporthalean taxa samples collected from diseased leaves of Cinnamomum camphora (Lauraceae), Castanopsis fordii (Fagaceae) and Schima superba (Theaceae) in Fujian province, China. Based on morphological features coupled to multigene phylogenetic analyses of the internal transcribed spacer (ITS) region, the large subunit of nuclear ribosomal RNA (LSU), the partial beta-tubulin (tub2), histone H3 (his3), DNA-directed RNA polymerase II subunit (rpb2), translation elongation factor 1-α (tef1) and calmodulin (cal) genes, three new species of Diaporthales are introduced, namely, Diaporthe wuyishanensis, Gnomoniopsis wuyishanensis and Paratubakia schimae. This study contributes to our understanding on the biodiversity of diaporthalean fungi that are inhabitants of the phyllosphere of trees native to Asia.

Keywords: Diaporthe; Paratubakia; multigene phylogeny; new species; taxonomy.

Figure 4

Diaporthe wuyishanensis (HMAS 352949). (a) Diseased leaves of Cinnamomum camphora; (b,c) surface and reverse sides of colony after 7 days on PDA (d,e) and 14 days; (f,g) conidiomata; (h) conidiogenous cells and conidia; and (i,j) alpha conidia. Scale bars: (h–j) 10 µm.

Figure 5

Gnomoniopsis wuyishanensis (HMAS 353149). (a) Diseased leaves of Castanopsis fordii; (b) surface and reverse sides of colony after 7 days on PDA (c) and 14 days; (d,e) conidiomata; (f–k) conidiogenous cells and conidia; and (l,m) conidia. Scale bars: (f–m) 10 µm.

Figure 6

Paratubakia schimae (HMAS 353150). (a) Diseased leaves of Schima superba; (b) surface and reverse sides of colony after 7 days on PDA (c) and 14 days; (d,e) conidiomata; (f–j) conidiogenous cells and conidia; and (k,l) conidia. Scale bars: (f–l) 10 µm.

Figure 1

Consensus tree of Diaporthe virgiliae species complex inferred from Bayesian inference analyses based on the combined ITS, cal, his3, tef1 and tub2 sequence dataset, with Diaporthe shennongjiaensis (CNUCC 201905) as the outgroup. The Maximum likelihood (ML) bootstrap support values and Bayesian posterior probabilities (BPPs) above 80% and 0.90 are shown at the nodes. Strains marked with “T” are ex-type, ex-epitype and ex-neotype. The isolates from this study are indicated in red.

Figure 2

Consensus tree of Gnomoniopsis inferred from Bayesian inference analyses based on the combined ITS, tef1 and tub2 sequence dataset, with Melanconis stilbostoma (CBS 109778) as the outgroup. The Maximum likelihood (ML) bootstrap support values and Bayesian posterior probabilities (BPPs) above 80% and 0.90 were shown at the nodes. Strains marked with “T” are ex-type, ex-epitype and ex-neotype. The isolates from this study are indicated in red.

Figure 3

Consensus tree of Tubakiaceae inferred from Bayesian inference analyses based on the combined ITS, LSU, rpb2, tef1 and tub2 sequence dataset, with Greeneria uvicola (FI12007) as the outgroup. The Maximum likelihood (ML) bootstrap support values and Bayesian posterior probabilities (BPPs) above 80% and 0.90 were shown at the nodes. Strains marked with “T” are ex-type, ex-epitype and ex-neotype. The isolates from this study are indicated in red.