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. 1985 Jan;144(1):189-92.
doi: 10.1016/0003-2697(85)90103-4.

Concentration and purification of influenza virus from allantoic fluid

D J Arora, P Tremblay, R Bourgault, S Boileau

Concentration and purification of influenza virus from allantoic fluid

D J Arora et al. Anal Biochem. 1985 Jan.

Abstract

A simple procedure which enables the concentration and purification of influenza virus, using an angular rotor, is described. Virus is concentrated over a sucrose step gradient. The same gradients are reused and volumes up to 4 liters are concentrated in 1 day. The concentrated virus is further purified by a simplified density-gradient technique. No host cell protein is detectable in the final product. The technique offers a broad application potential for concentrating and purifying other viruses.

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