The effect of sciatic nerve transection on myelinated fibers in the L5 dorsal root and lumbar dorsal column. A Marchi study in the rat

Abstract

The occurrence of Marchi-positive structures (MPS) in the L5 dorsal root and lumbar dorsal column was examined 1-18 weeks after unilateral sciatic nerve transection in rats, and compared to the occurrence of MPS during Wallerian degeneration seen after transection of L4 and L5 dorsal roots. There was an increasing number of MPS centrally to the junction between the peripheral (PNS) and central nervous system (CNS) and in the lumbar dorsal column ipsilateral to sciatic nerve transection throughout the examined time period. In the portion of the root distal to the PNS-CNS junction MPS were rare before 12 weeks postoperatively after which time small groups of MPS appeared. At all stages the incidence of MPS was just a fraction of that seen during Wallerian degeneration. From these observations it is inferred that few ganglion cells with myelinated central processes undergo complete disintegration after peripheral nerve transection. In addition, some of the myelinated central ganglion cell processes appear to be more severely affected proximal to the PNS-CNS junction than distally to it.