Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Dec 30;14(1):16.
doi: 10.3390/pathogens14010016.

First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status

Magdalena Materniak-Kornas  1 , Katarzyna Piórkowska  2 , Katarzyna Ropka-Molik  2 , Adrianna Dominika Musiał  2 , Joanna Kowalik  1 , Anna Kycko  3 , Jacek Kuźmak  1
Affiliations

First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status

Magdalena Materniak-Kornas et al. Pathogens. .

Abstract

Small ruminant lentiviruses (SRLVs) infect sheep, causing a multiorganic disease called maedi-visna or ovine progressive pneumonia, which significantly affects the production and welfare of sheep, generating serious economic losses. Although not all infected animals develop fully symptomatic disease, they constantly spread the virus in the flock. Since the infection is incurable and no vaccine is available, another approach is necessary to control SRLV infections. In recent years, an alternative for culling infected animals has become the approach based on identifying genetic markers for selecting SRLV-resistant individuals. Recent reports revealed several candidates, including gene encoding transmembrane protein 154 (TMEM154). Several single nucleotide polymorphisms (SNPs) are found within this gene in sheep of different breeds, but only some can be considered as resistant markers. This study aimed to investigate the presence of single polymorphic sites in TMEM154 gene in sheep of selected Polish flocks and assess their association with the infection and proviral load in the context of susceptibility to SRLV infection. In total 107 sheep, representing three breeds, were screened for their SRLV infection status by serological and PCR testing. All these animals were also genotyped to characterize the presence of SNPs in TMEM154 gene and estimate their potential of being the SRLV-resistance marker. The frequency of identified alleles differed among breeds. Moreover, the positive association between TMEM154 genotype and SRLV status was found for E35K polymorphism and two polymorphic sites in 5'UTR in one of analyzed breed. However, when the relationship between SNPs and SRLV proviral load was analyzed, five had a strong association, considering the whole population of tested sheep. Presented data, for the first time, identified the presence of SNPs in TMEM154 gene in sheep housed in Polish flocks and suggested that selecting SRLV-resistant animals based on this analysis might be possible, but further validation in a larger group of sheep is required.

Keywords: TMEM154; genotyping; sheep; single nucleotide polymorphism; small ruminant lentiviruses (SRLVs).

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Distribution of the SRLV copy numbers in sheep from three breeds. The grey dots present the provirus copy number of each sheep, the black dashed line represents the mean of the SRLV copy number in each breed, and the orange error bars represent the 95% CI for the mean.
Figure 2
Figure 2
The association of TMEM154 genotypes with SRLV proviral load.
Figure 3
Figure 3
The association of selected TMEM154 diplotypes (according to Table S3) with SRLV proviral load.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Colitti B., Coradduzza E., Puggi oni G., Capucchio M.T., Reina R., Bertolotti L., Rosati S. A new approach for Small Ruminant Lentivirus full genome characterization revealed the circulation of divergent strains. PLoS ONE. 2019;14:e0212585. doi: 10.1371/journal.pone.0212585. - DOI - PMC - PubMed
    1. Minguijón E., Reina R., Pérez M., Polledo L., Villoria M., Ramírez H., Leginagoikoa I., Badiola J.J., García-Marín J.F., de Andrés D., et al. Small ruminant lentivirus infections and diseases. Vet. Microbiol. 2015;181:75–89. doi: 10.1016/j.vetmic.2015.08.007. - DOI - PubMed
    1. Blacklaws B.A., Berriatua E., Torsteinsdottir S., Watt N.J., de Andres D., Klein D., Harkiss G.D. Transmission of small ruminant lentiviruses. Vet. Microbiol. 2004;101:199–208. doi: 10.1016/j.vetmic.2004.04.006. - DOI - PubMed
    1. Peterhans E., Greenland T., Badiola J., Harkiss G., Bertoni G., Amorena B., Eliaszewicz M., Juste R.A., Krassnig R., Lafont J.-P., et al. Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes. Vet. Res. 2004;35:257–274. doi: 10.1051/vetres:2004014. - DOI - PubMed
    1. Furtado Araújo J., Andrioli A., Pinheiro R.R., Sider L.H., de Sousa A.L.M., de Azevedo D.A.A., Peixoto R.M., Lima A.M.C., Damasceno E.M., Souza S.C.R., et al. Vertical transmissibility of small ruminant lentivirus. PLoS ONE. 2020;15:e0239916. doi: 10.1371/journal.pone.0239916. - DOI - PMC - PubMed

Substances

LinkOut - more resources