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Review
. 2025 Jan 25;163(1):27.
doi: 10.1007/s00418-025-02356-7.

Differentiation of stem cells into chondrocytes and their potential clinical application in cartilage regeneration

Julia Cieśla  1 , Marcin Tomsia  2
Affiliations
Review

Differentiation of stem cells into chondrocytes and their potential clinical application in cartilage regeneration

Julia Cieśla et al. Histochem Cell Biol. .

Abstract

Cartilage diseases and injuries are considered difficult to treat owing to the low regenerative capacity of this tissue. Using stem cells (SCs) is one of the potential methods of treating cartilage defects and creating functional cartilage models for transplants. Their ability to proliferate and to generate functional chondrocytes, a natural tissue environment, and extracellular cartilage matrix, makes SCs a new opportunity for patients with articular injuries or incurable diseases, such as osteoarthritis (OA). The review summarizes the most important scientific reports on biology and mechanisms of SC-derived chondrogenesis and sources of SCs for chondrogenic purposes. Additionally, it focuses on the genetic mechanisms, microRNA (miRNA) regulation, and epigenetic processes steering the chondrogenic differentiation of SCs. It also describes the attempts to create functional cartilage with tissue engineering using growth factors and scaffolds. Finally, it presents the challenges that researchers will have to face in the future to effectuate SC differentiation methods into clinical practice for treating cartilage diseases.

Keywords: Chondrocytes; Chondrogenesis; Stem cells; Tissue engineering.

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Conflict of interest statement

Declarations. Conflict of interest: The authors have no conflicts of interest to declare that are relevant to the content of this article.

References

    1. Adam EN, Janes J, Lowney R, Lambert J, Thampi P, Stromberg A, MacLeod JN (2019) Chondrogenic differentiation potential of adult and fetal equine cell types. Vet Surg 48(3):375–387. https://doi.org/10.1111/vsu.13183 - DOI - PubMed
    1. Aisenbrey EA, Bryant SJ (2019) The role of chondroitin sulfate in regulating hypertrophy during MSC chondrogenesis in a cartilage mimetic hydrogel under dynamic loading. Biomaterials 190:51–62. https://doi.org/10.1016/j.biomaterials.2018.10.028 - DOI - PubMed
    1. Apelgren P, Amoroso M, Lindahl A, Brantsing C, Rotter N, Gatenholm P, Kölby L (2017) Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo. PLoS ONE 12(12):e0189428. https://doi.org/10.1371/journal.pone.0189428 - DOI - PubMed - PMC
    1. Augustyniak E, Trzeciak T, Richter M, Kaczmarczyk J, Suchorska W (2015) The role of growth factors in stem cell-directed chondrogenesis: a real hope for damaged cartilage regeneration. Int Orthop 39(5):995–1003. https://doi.org/10.1007/s00264-014-2619-0 - DOI - PubMed
    1. Bae HC, Park HJ, Wang SY, Yang HR, Lee MC, Han HS (2018) Hypoxic condition enhances chondrogenesis in synovium-derived mesenchymal stem cells. Biomater Res 22(1):1–8. https://doi.org/10.1186/s40824-018-0134-x - DOI

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