Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2025 Aug;72(4):1121-1134.
doi: 10.1002/bab.2713. Epub 2025 Jan 26.

Evaluation of the Role of PnuC Gene in Enhancing Nicotinamide Mononucleotide Synthesis

Giang Thi Thu Vo  1 , Khang Khoa Hoang Nguyen  1 , Beom Soo Kim  1
Affiliations
Review

Evaluation of the Role of PnuC Gene in Enhancing Nicotinamide Mononucleotide Synthesis

Giang Thi Thu Vo et al. Biotechnol Appl Biochem. 2025 Aug.

Abstract

The PnuC gene plays a crucial role in the complex processes related to the absorption and synthesis of the nicotinamide mononucleotide (NMN) precursor. NMN, a nicotinamide adenine dinucleotide (NAD+) precursor, is important for cellular energy metabolism, DNA repair, and antiaging. This study focuses on elucidating the precursor absorption mechanism and the specific function of the PnuC gene in encoding membrane transport proteins, as well as its impact on the regulation and dynamics of NMN within the cell. This understanding aims to provide insights into its potential effects on metabolic balance, illustrated through two NAD+ biosynthesis pathways based on renewable and readily available cytoplasmic resources, assessing the potential of PnuC gene expression in clarifying complex interactions within regulation mechanisms. Enhanced expression analysis of the PnuC gene has initiated discussions on its potential applications in treating aging-related diseases and dysfunctions, contributing to cellular health maintenance.

Keywords: PnuC gene; cellular metabolism; nicotinamide adenine dinucleotide; nicotinamide mononucleotide.

PubMed Disclaimer

References

    1. H. Li, Y. Yang, W. Hong, M. Huang, M. Wu, and X. Zhao, “Applications of Genome Editing Technology in the Targeted Therapy of Human Diseases: Mechanisms, Advances and Prospects,” Signal Transduction and Targeted Therapy 5 (2020): 1, https://doi.org/10.1038/s41392‐019‐0089‐y.
    1. E. Sauer, M. Merdanovic, A. Price Mortimer, G. Bringmann, and J. Reidl, “PnuC and the Utilization of the Nicotinamide Riboside Analog 3‐Aminopyridine in Haemophilus influenzae,” Antimicrobial Agents and Chemotherapy 48, no. 12 (2004): 4532–4541, https://doi.org/10.1128/AAC.48.12.4532‐4541.2004.
    1. S. Luo, J. Zhao, Y. Zheng, T. Chen, and Z. Wang, “Biosynthesis of Nicotinamide Mononucleotide: Current Metabolic Engineering Strategies, Challenges, and Prospects,” Fermentation 9 (2023): 594, https://doi.org/10.3390/fermentation9070594.
    1. H. Nadeeshani, J. Li, T. Ying, B. Zhang, and J. Lu, “Nicotinamide Mononucleotide (NMN) as an Anti‐aging Health Product—Promises and Safety Concerns,” Journal of Advanced Research 37 (2022): 267–278, https://doi.org/10.1016/j.jare.2021.08.003.
    1. G. C. Marinescu, R. G. Popescu, and A. Dinischiotu, “Size Exclusion Chromatography Method for Purification of Nicotinamide Mononucleotide (NMN) From Bacterial Cells,” Scientific Reports 8, no. (2018): 4433. https://doi.org/10.1038/s41598‐018‐22806‐8.

MeSH terms

Substances

LinkOut - more resources