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. 2024 Nov 16;43(6):828-834.
doi: 10.5937/jomb0-45950.

Clinical significance of NLRP3 inflammasome and related cell molecules in early diabetic kidney disease in elderly population

Xiaoli Li  1 , Shiwei Liu  1 , Jinrong Huangfu  1 , Nannan Lai  1 , Yan Shang  2
Affiliations

Clinical significance of NLRP3 inflammasome and related cell molecules in early diabetic kidney disease in elderly population

Xiaoli Li et al. J Med Biochem. .

Abstract
in English, Serbian

Background: The paper aims to investigate the expression level of NLRP3 inflammasome and its related cell molecules in early diabetes kidney disease (EDKD) in the elderly and its clinical application value.

Methods: From October 2021 to April 2023, 50 elderly patients with T2DM (T2DM group), 50 elderly patients with EDKD (EDKD group) and 50 elderly people who passed the health check-up (healthy group) were chosen as the study subjects. Plasma NLRP3 inflammasome and related cells (blood leukocyte count, monocyte count, lymphocyte count) molecular (NT-proBNP and others) levels are tested, and Pearson correlation analysis is utilized to explore the correlation among plasma NLRP3 inflammasome and related cells, molecules, and renal function indicators (UACR, BUN, Ucr) in elderly patients with EDKD.

Results: (1) The three groups' comparison in HbA1c, FIns, HOMA-IR, UACR, BUN, Ucr, SOD, MCP-1, and TNF-a levels were with P<0.05. The levels of TG and LDL-C in the EDKD group were higher than those in the T2DM and the healthy groups; the levels of FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-a in the EDKD and T2DM groups were higher than those in the healthy group, while SOD was smaller than that in the healthy group; the levels of BUN, Ucr, hs-CRP, FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-a in the EDKD group were higher than those in the T2DM group, while SOD was smaller than that in the T2DM group. The above results were with P<0.05. (2) It has P<0.05 in Monocyte count, NLRP3, NT-proBNP, caspase-1, ASC and others in the three groups. Those in the EDKD and T2DM groups were higher than those in the healthy group. The levels of these indicators in the EDKD group were higher than those in the T2DM group, with P<0.05. NLRP3, Caspase-1, ASC, IL1b, and IL-18 were positively correlated with UACR, BUN, and Ucr in the EDKD group. All the above differences were P<0.05.

Conclusions: NLRP3 inflammasome and its related molecules caspase-1, ASC, IL-1b, IL-18 and other levels increase in early elderly EDKD and are closely related to the severity of EDKD.

Uvod: Rad ima za cilj da ispita nivo ekspresije NLRP3 inflamazoma i njemu srodnih ćelijskih molekula u ranoj fazi dijabetesne bubrežne bolesti (EDKD) kod starijih osoba i njegovu kliničku primenu.

Metode: Od oktobra 2021. do aprila 2023. godine, izabrano je 50 starijih pacijenata sa T2DM (T2DM grupa), 50 starijih pacijenata sa EDKD (EDKD grupa) i 50 starijih osoba koje su prošle zdravstveni pregled (zdrava grupa). Testirani su nivoi inflamazoma NLRP3 u plazmi i srodni (broj leukocita u krvi, broj monocita, limfocita) molekularni (NT-proBNP i drugi) nivoi, a korišćena je Pirsonova korelaciona analiza za istraživanje korelacije između plazma NLRP3 inflamazoma i srodnih ćelija, molekula i indikatora renalne funkcije (UACR, BUN, Ucr) kod starijih pacijenata sa EDKD.

Rezultati: (1) Poređenje tri grupe u nivoima HbA1c, FIns, HOMA-IR, UACR, BUN, Ucr, SOD, MCP-1 i TNF-a bilo je sa P<0,05. Nivoi TG i LDL-C u grupi sa EDKD su bili viši od onih u T2DM i zdravim grupama; nivoi FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-a u grupama EDKD i T2DM su bili viši od onih u zdravoj grupi, dok je SOD bio manji od onog u zdravoj grupi; nivoi BUN, Ucr, hs-CRP, FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-a u EDKD grupi su bili viši od onih u T2DM grupi, dok je SOD bio manji od nivoa u grupi T2DM. Gornji rezultati su bili sa P<0,05. (2) Ima P<0,05 u broju monocita, NLRP3, NT-proBNP, kaspazi-1, ASC i drugima u tri grupe. Oni u EDKD i T2DM grupama su bili veći od onih u zdravoj grupi. Nivoi ovih indikatora u grupi sa EDKD su bili viši od onih u grupi sa T2DM, sa P<0,05. NLRP3, kaspaza-1, ASC, IL-1b i IL-18 su bili u pozitivnoj korelaciji sa UACR, BUN i Ucr u EDKD grupi. Sve gore navedene razlike su P<0,05.

Zaključak: NLRP3 inflamazom i njemu srodni molekuli kaspaza-1, ASC, IL-1b, IL-18 i drugi nivoi se povećavaju u ranoj dijabetesnoj bolesti bubrega kod starije populacije i usko su povezani sa ozbiljnošću EDKD.

Keywords: NLRP3 inflammasome; clinical significance; early diabetes; kidney disease.

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Conflict of interest statement

All the authors declare that they have no conflict of interest in this work.Conflict of Interest: The authors stated that they have no conflicts of interest regarding the publication of this article.

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