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. 1985 Apr 15;41(4):456-8.
doi: 10.1007/BF01966144.

Muscle fluorometry: a determination of the depth of penetration

A W Stewart

Muscle fluorometry: a determination of the depth of penetration

A W Stewart. Experientia. .

Abstract

Fluorometric recordings of NADH (nicotinamide adenine dinucleotide) were made on rabbit papillary muscles. The specimens were placed between the UV light source and a small window of the detection stage. As the muscle was moved over the window in a transverse direction, simultaneous measurements could be taken of transmitted UV light and fluorescent light for various thicknesses of tissue. It is concluded that a muscle thickness of 0.65 mm is optimal before absorption of the incident light decreases the fluorescence signal.

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