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Review
. 2024 Sep 26;2(4):34.
doi: 10.1007/s44307-024-00041-9.

Advancements in delivery strategies and non-tissue culture regeneration systems for plant genetic transformation

Minyi Wu  1   2 , Ao Chen  1   2 , Xiaomeng Li  3 , Xiaoyun Li  3 , Xingliang Hou  4   5 , Xu Liu  6   7
Affiliations
Review

Advancements in delivery strategies and non-tissue culture regeneration systems for plant genetic transformation

Minyi Wu et al. Adv Biotechnol (Singap). .

Abstract

Plant genetic transformation is a pivotal and essential step in modifying important agronomic traits using biotechnological tools, which primarily depend on the efficacy of transgene delivery and the plant regeneration system. Over the years, advancements in the development of delivery methods and regeneration systems have contributed to plant engineering and molecular breeding. Recent studies have demonstrated that the efficiency of plant transformation can be improved by simultaneously delivering meristem-developmental regulators, utilizing virus-mediated gene editing, and executing non-sterile in planta manipulations. Efficient genetic delivery and non-tissue culture regeneration systems are gradually being developed. This review summarizes diverse delivery strategies and in planta regeneration techniques aimed at improving the efficiency of plant genetic transformation. We also emphasize the integration and utilization of these emerging transgenic approaches for expediting future crop engineering.

Keywords: In planta transformation; Delivery strategy; Non-tissue culture; Plant genetic transformation; Plant regeneration system.

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Conflict of interest statement

Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: All authors approved the final manuscript and the submission to this journal. Competing interests: The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Timeline of delivery systems for plant genetic transformation. Note: DRs, developmental regulators; CBD, cut–dip–budding delivery method; RAIPD, Regenerative Activity-dependent in Planta Injection Delivery
Fig. 2
Fig. 2
Tissue culture and non-tissue culture regeneration systems for plant transformation
See this image and copyright information in PMC

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