Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025:2895:165-176.
doi: 10.1007/978-1-0716-4350-1_12.

Identification of Lignans

Hye Mi Kim  1 , Chul Young Kim  2
Affiliations

Identification of Lignans

Hye Mi Kim et al. Methods Mol Biol. 2025.

Abstract

Lignans have garnered significant interest in various fields of pharmaceuticals, nutrition, and pesticides due to their diverse biological activities. This chapter primarily focuses on the extraction and purification methods of lignans from Schisandra, followed by an extensive examination of qualitative and quantitative analytical techniques, including thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Additionally, methods for structural analysis using nuclear magnetic resonance (NMR) are briefly summarized. The chapter also details approaches for determining the relative and absolute composition of lignan compounds, with illustrative examples provided.

Keywords: Chromatography; Dibenzocyclooctadiene lignans; HPLC; Lignans; NMR; Schisandra chinensis; TLC.

PubMed Disclaimer

References

    1. Robbers, J. E., Speedie, M. K., Tyler, V. E. (1996). Pharmacognosy and pharmacobiotechnology
    1. Badal S, Delgoda R (eds) (2017) Pharmacognosy. Fundamentals, applications and strategy. Academic Press, Amsterdam
    1. Yu X, Che X, Xu H (2017) Recent advances in the chemistry and biology of podophyllotoxins. Chem Eur J 23(19):4467–4526. https://doi.org/10.1002/chem.201602472 - DOI - PubMed
    1. Kamal A, Reddy TS, Polepalli S, Shalini N, Reddy VG, Rao AS, Jain N, Shankaraiah N (2014) Synthesis and biological evaluation of podophyllotoxin congeners as tubulin polymerization inhibitors. Bioorg Med Chem 22(19):5466–5475. https://doi.org/10.1016/j.bmc.2014.07.031 - DOI - PubMed
    1. Willför SM, Smeds AI, Holmbom BR (2006) Chromatographic analysis of lignans. J Chromatogr A 1112(1–2):64–77. https://doi.org/10.1016/j.chroma.2005.11.054 - DOI - PubMed

MeSH terms

LinkOut - more resources