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. 2025 Feb;113(2):e35536.
doi: 10.1002/jbm.b.35536.

Simvastatin-Loaded Chitosan Microspheres as a Biomaterial for Dentin Tissue Engineering

Erika Soares Bronze-Uhle  1 , Camila Correa da Silva Braga de Melo  1 , Isabela Sanches Pompeo da Silva  1 , Vitor de Toledo Stuani  1 , Victor Hugo Bueno  2 , Daniel Rinaldo  2 , Carlos Alberto de Souza Costa  3 , Paulo Noronha Lisboa Filho  4 , Diana Gabriela Soares  1
Affiliations

Simvastatin-Loaded Chitosan Microspheres as a Biomaterial for Dentin Tissue Engineering

Erika Soares Bronze-Uhle et al. J Biomed Mater Res B Appl Biomater. 2025 Feb.

Abstract

In the present study, chitosan microspheres (MSCH) loaded with different concentrations of simvastatin (2%, 5%, and 10%) were synthesized as a biomaterial for dentin tissue engineering. The microspheres were prepared by emulsion crosslinking method, and simvastatin was incorporated during the process. The microspheres were then physicochemically and morphologically characterized. Scanning electron microscopy and infrared spectroscopy confirmed the spherical morphology of synthesized microspheres and the chemical incorporation of simvastatin into MSCH, respectively. UV-visible absorption confirmed the controlled and continuous release pattern of the drug. To mimic the clinical application in vitro, the microspheres were applied onto three-dimensional (3D) cultures of human dental pulp cells (HDPCs). Cell viability, proliferation, and in situ-mineralized matrix deposition were evaluated. The results indicated no cytotoxic effects for all 3D cultures for all tested biomaterials, with cells being able to proliferate significantly over time. HDPCs showed a significant increase in the deposition of mineralization nodules when 3D cultures were in direct contact with chitosan microspheres in comparison to control; nevertheless, the highest expression was observed for MSCH encapsulated with 5% and 10% simvastatin, which was significantly higher than plain MSCH. Therefore, chitosan microsphere systems loaded with 5%-10% simvastatin provided the development of a controlled release system in bioactive dosages for dentin tissue engineering.

Keywords: chitosan microspheres; dentin tissue engineering; drug delivery; human dental pulp cells; regenerative dentistry; simvastatin.

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References

    1. L. Wang, Y. Liu, W. Zhang, X. Chen, T. Yang, and G. Ma, “Microspheres and Microcapsules for Protein Delivery: Strategies of Drug Activity Retention,” Current Pharmaceutical Design 19, no. 35 (2013): 6340–6352.
    1. P. Chocholata, V. Kulda, and V. Babuska, “Fabrication of Scaffolds for Bone‐Tissue Regeneration,” Materials 12, no. 4 (2019): 568–593.
    1. B. Fonseca‐Santos and M. Chorilli, “An Overview of Carboxymethyl Derivatives of Chitosan: Their Use as Biomaterials and Drug Delivery Systems,” Materials Science & Engineering. C, Materials for Biological Applications 77 (2017): 1349–1362.
    1. L. Roseti, V. Parisi, M. Petretta, et al., “Scaffolds for Bone Tissue Engineering: State of the Art and New Perspectives,” Materials Science & Engineering. C, Materials for Biological Applications 78 (2017): 1246–1262.
    1. C. Y. Wee, Z. J. Yang, and E. S. Thian, “Past, Present and Future Development of Microspheres for Bone Tissue Regeneration: A Review,” Materials Technology 36, no. 6 (2021): 364–374.

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