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[Preprint]. 2025 Jan 26:2025.01.23.634600.
doi: 10.1101/2025.01.23.634600.

Feature-Driven Whole-Tissue Imaging with Subcellular Resolution

Jinlong Lin, Zach Marin, Xiaoding Wang, Hazel M Borges, Pierre-Emmanuel Y N'Guetta, Xuemei Luo, Baylee A Porter, Yuanyuan Xue, Md Torikul Islam, Tai Ngo, Arin B Aurora, Hu Zhao, Suzanne D Conzen, Sean J Morrison, Shuang Liang, Zhenyu Zhong, Lori L O'Brien, Kevin M Dean

Feature-Driven Whole-Tissue Imaging with Subcellular Resolution

Jinlong Lin et al. bioRxiv. .

Update in

  • Feature-driven whole-tissue imaging with subcellular resolution.
    Lin J, Marin Z, Wang X, Borges HM, Shen Q, N'Guetta PY, Luo X, Porter BA, Xue Y, Islam MT, Ngo T, Idonije D, Gałecki S, Aurora AB, Zhao H, Conzen SD, Morrison SJ, Liang S, Zhong Z, O'Brien LL, Dean KM. Lin J, et al. Cell Rep Methods. 2025 Sep 15;5(9):101148. doi: 10.1016/j.crmeth.2025.101148. Epub 2025 Sep 2. Cell Rep Methods. 2025. PMID: 40902591

Abstract

Understanding how subcellular structures and events shape tissue-wide phenomena remains a central challenge in biology. We present Multiscale Cleared Tissue Axially Swept Light-Sheet Microscopy (MCT-ASLM), a platform combining cm-scale imaging with targeted high-resolution interrogation of intact tissues in human-guided or autonomous modes. Capable of capturing fields of view up to 21 mm at micron-scale resolution, MCT-ASLM can seamlessly transition to submicron (~300 nm) isotropic resolution for targeted imaging. This versatility enables comprehensive studies of hierarchical organization and spatially complex processes, including mapping neuronal circuits in rat brains, visualizing glomerular innervation in mouse kidneys, and examining metastatic tumor microenvironments. By bridging subcellular to tissue-level scales, MCT-ASLM offers a powerful method for unraveling how local events contribute to global biological phenomena.

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