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. 1985 Feb;10(2):269-82.
doi: 10.1007/BF00964573.

Use of mitochondrial inhibitors to differentiate kinetic properties of the ATP-dependent Ca2+ uptake system in synaptic membranes

Use of mitochondrial inhibitors to differentiate kinetic properties of the ATP-dependent Ca2+ uptake system in synaptic membranes

D H Ross et al. Neurochem Res. 1985 Feb.

Abstract

Synaptosomal membranes accumulate 3-6 times more Ca2+ in the presence of ATP (50-1000 microM) than basal Ca2+ accumulation (-ATP). The location of this Ca2+ accumulation appears to reside on the cytosolic face of the synaptosome since lysed synaptosomes accumulate 4-times more Ca2+ than intact synaptosomes. The inclusion of mitochondrial inhibitors, oligomycin (0.7 micrograms/ml), sodium azide (100 microM) and dinitrophenol (100 microM) differentiate mitochondrial from nonmitochondrial Ca2+ accumulation under conditions that are [Ca2+]- and ATP-dependent. In the presence of low concentrations of ATP (less than 150 microM) and Ca2+ free (2.5 or 6.8 microM), Ca2+ accumulation occurs as one process in both lysed synaptosomal membranes and purified synaptic plasma membranes in the presence and/or absence of MI. When ATP levels are increased (greater than 200 microM), Ca2+ accumulation process remains independent of the presence of mitochondrial inhibitors when Ca2+ free = 2.5 microM. When Ca2+ free is increased to 6.8 microM, mitochondrial inhibitors differentiate mitochondrial from nonmitochondrial accumulation. These studies suggest that optimal conditions for the measurement of Ca2+ accumulating mechanisms in synaptosomal membranes depend on both [Ca2+] and ATP. Use of these assay conditions provide evidence that ATP-dependent Ca2+ uptake may be a viable mechanism for the regulation of synaptosomal Ca2+ levels.

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