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. 2025 May;65(5):e70006.
doi: 10.1002/jobm.70006. Epub 2025 Feb 12.

Cultivation Strategies to Improve Chlamydomonas reinhardtii Growth and Recombinant Mcherry Expression

Jassiara da Silva Pessoa  1 , Bruno Guzzo da Silva  1 , Ednilson Donisete de França Júnior  1 , Isac José da Silva Filho  1 , João Vitor Dutra Molino  2 , João Carlos Monteiro de Carvalho  3 , Livia Seno Ferreira-Camargo  1
Affiliations

Cultivation Strategies to Improve Chlamydomonas reinhardtii Growth and Recombinant Mcherry Expression

Jassiara da Silva Pessoa et al. J Basic Microbiol. 2025 May.

Abstract

Chlamydomonas reinhardtii is a promising model microalga for recombinant molecules production. Nonetheless, low yield is a challenge for its industrial use. This work investigated the influence of ammonium chloride (NH4Cl) concentration and temperature on the growth of transgenic C. reinhardtii expressing the fluorescent protein mCherry on a laboratory scale. A Central Composite Rotatable Design was used to establish the cultivation conditions. NH4Cl concentrations ranging from 400 to 647.49 mg/L and temperatures between 25°C and 32.1°C resulted in maximum values of cell concentration and mCherry fluorescence. Lower temperatures (15°C-17°C) were found to be more suitable for the accumulation of total soluble proteins. These results demonstrate that cultivation conditions can positively affect C. reinhardtii growth, with a range of conditions that can be used. Unlike genetic approaches, this study provides a solution to enhance both growth and recombinant protein expression in C. reinhardtii. These findings pave the way for scaling up the use of C. reinhardtii as a biofactory in industry and can be applied to other microalgal systems.

Keywords: CCRD; Chlamydomonas reinhardtii; fluorescent protein; growth optimization; mCherry; transgenic microalga.

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References

    1. E. W. Becker, “Micro‐Algae as a Source of Protein,” Biotechnology Advances 25 (2007): 207–210.
    1. B. A. Rasala and S. P. Mayfield, “Photosynthetic Biomanufacturing in Green Algae; Production of Recombinant Proteins for Industrial, Nutritional, and Medical Uses,” Photosynthesis Research 123 (2015): 227–239.
    1. L. Tomaselli, Handbook of Microalgal Culture, eds. A. Richmond (Oxford, UK: Blackwell Publishing Ltd, 2003. 1, 1–19).
    1. D. Vlček, A. Ševčovičová, B. Sviežená, E. Gálová, and E. Miadoková, “Chlamydomonas Reinhardtii: A Convenient Model System for the Study of Dna Repair in Photoautotrophic Eukaryotes,” Current Genetics 53 (2008): 1–22.
    1. R. Delosme, J. Olive, and F.‐A. Wollman, “Changes in Light Energy Distribution Upon State Transitions: an In Vivo Photoacoustic Study of the Wild Type and Photosynthesis Mutants From Chlamydomonas Reinhardtii,” Biochimica et Biophysica Acta (BBA) ‐ Bioenergetics 1273 (1996): 150–158.

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