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Review
. 2025 Jan 22;14(3):170.
doi: 10.3390/cells14030170.

Cellular Systems for Colorectal Stem Cancer Cell Research

Tatyana A Grigoreva  1 , Daria N Kindt  1 , Aleksandra V Sagaidak  1 , Daria S Novikova  1 , Vyacheslav G Tribulovich  1
Affiliations
Review

Cellular Systems for Colorectal Stem Cancer Cell Research

Tatyana A Grigoreva et al. Cells. .

Abstract

Oncological diseases consistently occupy leading positions among the most life-threatening diseases, including in highly developed countries. At the same time, the second most common cause of cancer death is colorectal cancer. The current level of research shows that the development of effective therapy, in this case, requires a new grade of understanding processes during the emergence and development of a tumor. In particular, the concept of cancer stem cells that ensure the survival of chemoresistant cells capable of giving rise to new tumors is becoming widespread. To provide adequate conditions that reproduce natural processes typical for tumor development, approaches based on increasingly complex cellular systems are being improved. This review discusses the main strategies that allow for the study of the properties of tumor cells with an emphasis on colorectal cancer stem cells. The features of working with tumor cells and the advantages and disadvantages of 2D and 3D culture systems are considered.

Keywords: 3D cell culture; cancer stem cells (CSCs); colonosphere; organoid; spheroid.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Scheme of cancer stem cell proliferation and differentiation.
Figure 2
Figure 2
Types of cell structures used in colorectal cancer modeling.
See this image and copyright information in PMC

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