Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Apr;146(1):23-7.
doi: 10.1016/0003-2697(85)90389-6.

A method of isolating nick-translated DNA by subsequent separation on low-melting-temperature agarose

L S Libby, J H Fisher, C H Scoggin

A method of isolating nick-translated DNA by subsequent separation on low-melting-temperature agarose

L S Libby et al. Anal Biochem. 1985 Apr.

Abstract

The use of nick translation of cloned DNA segments followed by separation on low-melting-temperature agarose has been used to obtain multiple radiolabeled DNA probes from a single nick-translation procedure. This technique avoids gel matrix inhibition of enzymatic reactions. Examples of the utility of this procedure are presented and the advantages and drawbacks discussed.

PubMed Disclaimer

Publication types

LinkOut - more resources