Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jan 24;26(3):992.
doi: 10.3390/ijms26030992.

Infrared Laser-Assisted Extraction of Bioactive Compounds from Rosa canina L

Andreia Alecu  1 , Camelia Albu  1 , Georgiana-Ileana Badea  1 , Aurelia Alionte  1 , Alin-Alexandru Enache  2 , Gabriel-Lucian Radu  1 , Simona-Carmen Litescu  1
Affiliations

Infrared Laser-Assisted Extraction of Bioactive Compounds from Rosa canina L

Andreia Alecu et al. Int J Mol Sci. .

Abstract

The extraction of bio-compounds from medicinal plants provides opportunities for using the plant extract for health benefits. Rosa canina L. is considered a "natural superfood", and the valorization of its active compounds requires an extraction technique that ensures a suitable extraction yield while preserving the compounds' activity. In our study, infrared laser irradiation (IRLIR) technology was used for the first time in the bioactive compound's extraction from Rosa canina L. Different solvents (water-ethanol, hexane-ethanol) and different extraction times were tested to obtain a high extraction yield. Chromatographic and spectrophotometry methods were used to monitor the profile of bioactive compounds and the antioxidant activity of the extracts. The results obtained for IRLIR were compared with those obtained by accelerated solvent extraction (ASE), an advanced extraction method. The IRLIR technology proved to be a more reliable analytical tool for the extraction of (+)-catechin, gallic acid, and lutein. In addition, a richer extract formula was obtained by IRLIR extraction with respect to ASE, with the IRLIR process ensuring a short extraction time, low volume of the extraction solvent, low energy consumption, and a less expensive device.

Keywords: Rosa canina L.; accelerated solvent extraction; extraction technologies; infrared laser radiation extraction; lipophilic compounds; polyphenolic compounds.

PubMed Disclaimer

Conflict of interest statement

Author Alin-Alexandru Enache is the employee of the company S.C. Apel Laser S.R.L. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Chromatograms obtained for Rosa canina L. extract after IRLIRE in 80% ethanol (1—gallic acid, [M − H]− 169; 2—chlorogenic acid, [M − H]- 353; 3—(+)-catechin, [M − H]- 289; 4—rutin, [M − H]- 609; 5—ellagic acid, [M-H]- 301; 6—quercetin 3-β-D-glucoside, [M − H]- 463; 7—quercitrin, [M − H]- 447; 8—quercetin, [M − H]- 301) by HPLC-MS.
Figure 2
Figure 2
Chromatograms obtained for Rosa canina L. lipophilic extract after IRLIRE in ethanol–hexane mixture (1—lycopene; 2—lutein; 3—β-carotene; 4—α tocopherol) by HPLC-DAD.
Figure 3
Figure 3
The antioxidant capacity of standard polyphenolic compounds in 20% ethanol.
See this image and copyright information in PMC

References

    1. Chaves J.O., de Souza M.C., da Silva L.C., Lachos-Perez D., Torres-Mayanga P.C., Machado A.P.D.F., Forster-Carneiro T., Vázquez-Espinosa M., González-de-Peredo A.V., Barbero G.F., et al. Extraction of Flavonoids from Natural Sources Using Modern Techniques. Front. Chem. 2020;8:507887. doi: 10.3389/fchem.2020.507887. - DOI - PMC - PubMed
    1. Bitwell C., Indra S.S., Luke C., Kakoma M.K. A review of modern and conventional extraction techniques and their applications for extracting phytochemicals from plants. Sci. Afr. 2023;19:e01585. doi: 10.1016/j.sciaf.2023.e01585. - DOI
    1. Câmara J.S., Perestrelo R., Berenguer C.V., Andrade C.F.P., Gomes T.M., Olayanju B., Kabir A., Rocha C.M.R., Teixeira J.A., Pereira J.A.M. Green Extraction Techniques as Advanced Sample Preparation Approaches in Biological, Food, and Environmental Matrices: A Review. Molecules. 2022;27:2953. doi: 10.3390/molecules27092953. - DOI - PMC - PubMed
    1. Anastas P., Eghbali N. Green Chemistry: Principles and Practice. Chem. Soc. Rev. 2010;39:301–312. doi: 10.1039/B918763B. - DOI - PubMed
    1. Pirvu L.C., Nita S., Rusu N., Bazdoaca C., Neagu G., Bubueanu C., Udrea M., Udrea R., Enache A. Effects of Laser Irradiation at 488, 514, 532, 552, 660, and 785 nm on the Aqueous Extracts of Plantago lanceolata L.: A Comparison on Chemical Content, Antioxidant Activity and Caco-2 Viability. Appl. Sci. 2022;12:5517. doi: 10.3390/app12115517. - DOI

MeSH terms

LinkOut - more resources