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. 2025 Jan;178(3):351-359.
doi: 10.1007/s10517-025-06335-9. Epub 2025 Feb 13.

Relationship between Genes and microRNAs Involved in the Migration of Cells from the Bone Marrow during Experimental Liver Fibrosis

E I Lebedeva  1 , A T Shchastniy  2 , A S Babenka  3 , D A Zinovkin  4 , E A Nadyrov  4
Affiliations

Relationship between Genes and microRNAs Involved in the Migration of Cells from the Bone Marrow during Experimental Liver Fibrosis

E I Lebedeva et al. Bull Exp Biol Med. 2025 Jan.

Abstract

Progressive toxic liver fibrosis in Wistar male rats was characterized by the migration of CX3CR1+ and CD34+ cells from the bone marrow, accompanied by a mild increase in their numbers. From weeks 3 to 5 and 9 to 13, the number of CX3CR1+ cells remained approximately the same. The area occupied by CD34+ cells increased by 2 times (p<0.001) only by the end of the experiment. At week 3, the correlation between Cxcl12 and Notch2 mRNA was lost, while at week 9, a correlation of Cxcl12 with Notch1 and Notch2 was observed. From week 11 onwards, a correlation of Cxcl12 with Notch2 was revealed and a correlation with Notch1 disappeared. miR-3558-3p was correlated with Cxcl12 mRNA level at the stages of progressive fibrosis and nodular remodeling of the liver parenchyma. The greatest number of miRNAs showed direct and inverse correlations of moderate to medium strength, with Cxcl12 gene at the stage of complete cirrhosis. The mRNA levels of Cxcl12 and Yap1 showed a significant correlation with each other throughout the experiment. This suggests that they may be involved in the process of cell migration from the bone marrow to the liver and play a role in fibrosis and cirrhosis. They could be considered as potential targets for the development of new treatments.

Keywords: correlation analysis; experiment; immunohistochemistry; liver fibrogenesis; mRNA and microRNA expression.

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References

    1. Yang Y, Ni M, Zong R, Yu M, Sun Y, Li J, Chen P, Li C. Targeting Notch1-YAP circuit reprograms macrophage polarization and alleviates acute liver injury in mice. Cell Mol. Gastroenterol. Hepatol. 2023;15(5):1085-1104. https://doi.org/10.1016/j.jcmgh.2023.01.002 - DOI - PubMed - PMC
    1. Hu C, Wu Z, Li L. Mesenchymal stromal cells promote liver regeneration through regulation of immune cells. Int. J. Biol. Sci. 2020;16(5):893-903. https://doi.org/10.7150/ijbs.39725 - DOI - PubMed - PMC
    1. Wu X, Qian L, Zhao H, Lei W, Liu Y, Xu X, Li J, Yang Z, Wang D, Zhang Y, Zhang Y, Tang R, Yang Y, Tian Y. CXCL12/CXCR4: An amazing challenge and opportunity in the fight against fibrosis. Ageing Res. Rev. 2023;83:101809. https://doi.org/10.1016/j.arr.2022.101809 - DOI - PubMed
    1. Abubakr S, Hazem NM, Sherif RN, Elhawary AA, Botros KG. Correlation between SDF-1α, CD34 positive hematopoietic stem cells and CXCR4 expression with liver fibrosis in CCl4 rat model. BMC Gastroenterol. 2023;23(1):323. https://doi.org/10.1186/s12876-023-02932-y - DOI - PubMed - PMC
    1. Bianchi ME, Mezzapelle R. The chemokine receptor CXCR4 in cell proliferation and tissue regeneration. Front. Immunol. 2020;11:2109. https://doi.org/10.3389/fimmu.2020.02109 - DOI - PubMed - PMC

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